NDLI: Segmentation of sperms using the strategic hough transform

Content Provider	Springer Nature Link
Author	Park, Kwang Suk Yi, Won Jin Paick, Jae Seung
Copyright Year	1997
Abstract	A new method based on theHough transform has been used for the quantitative estimation of the morphological characteristics of the sperm. Images of the sperms were acquired into the digital format using the optical microscope, CCD camera, and frame grabber. For each sperm in the image, the region of interest for the segmentation of the sperm head was selected using the density difference between the sperm head and background. The boundary of the sperm head was approximated with an ellipse and represented by five parameters. These five parameters representing an ellipse have been investigated by applying the Hough transform strategically. During the searching procedue using the Hough transform, we restricted the searching volume minimally and moved the next searching volume to the direction showing the steepest gradient of the estimation error. Also, we increased the parameter resolution from the three pixel distance to the one pixel distance. For the tested images, head boundaries of the sperms were estimated with an error of 1/2 pixel distance, and morphological parameters were calculated by the equations without further image processing. This new method separates sperm head based on the geometrical characteristic of the sperm and calculates morphological parameters simply from the derived boundary.
Starting Page	294
Ending Page	302
Page Count	9
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	25
Issue Number	2
e-ISSN	15739686
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1997-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Sperm morphology Semen analysis Elliptic approximation Biomedicine general Biochemistry Mechanics Biophysics/Biomedical Physics Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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