NDLI: Knee-Loading Modality Drives Molecular Transport in Mouse Femur

Content Provider	Springer Nature Link
Author	Su, Min Jiang, Hui Zhang, Ping Liu, Yunlong Wang, Exing Hsu, Andrew Yokota, Hiroki
Copyright Year	2006
Abstract	Mechanical loading is well known to stimulate bone remodeling. Load-driven interstitial fluid flow and molecular transport have been postulated to play a role in the enhancement of bone formation. In order to evaluate load-driven molecular transport in a lacunocanalicular network, we conducted fluorescence recovery after photobleaching (FRAP) experiments using lacunae stained with uranine (376 Da). Loads were applied to a mouse femur ex vivo with a novel knee-loading modality, where the distal epiphysis was loaded with a sinusoidal force at 2 Hz. The lacunae in the diaphysis located 25% (∼4 mm) proximal to the loading site were photobleached and sequentially imaged, and a time constant for fluorescence recovery was determined both with and without knee loading. The time constant was estimated as the period to recover 63% of fluorescent intensity using a best-fit exponential curve. The results reveal that the applied loads shortened the time constant from 33 ± 9 s with non-loading control to 25 ± 11 s with knee loading (p = 0.0014). The strain in the measurement site was <100 μstain along the femoral midshaft, which was an order of magnitude smaller than the minimum effective strain threshold for bone remodeling. Taken together, the current study supports the notion that molecular transport in cortical bone is enhanced by the loads applied to the epiphysis without inducing significant in situ strain in the diaphysis.
Starting Page	1600
Ending Page	1606
Page Count	7
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	34
Issue Number	10
e-ISSN	15739686
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2006-09-20
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Mechanical loading Lacuna Fluorescent recovery after photobleaching Interstitial fluid flow Biochemistry Mechanics Biophysics/Biomedical Physics Biomedical Engineering Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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