NDLI: Mechanical Properties, Cytocompatibility and Manufacturability of Chitosan:PEGDA Hybrid-Gel Scaffolds by Stereolithography

Content Provider	Springer Nature Link
Author	Morris, Viola B. Nimbalkar, Siddharth Younesi, Mousa McClellan, Phillip Akkus, Ozan
Copyright Year	2016
Abstract	Extracellular matrix mimetic hydrogels which hybridize synthetic and natural polymers offer molecularly-tailored, bioactive properties and tunable mechanical strength. In addition, 3D bioprinting by stereolithography allows fabrication of internal pores and defined macroscopic shapes. In this study, we formulated a hybrid biocompatible resin using natural and synthetic polymers (chitosan and polyethylene glycol diacrylate (PEGDA), respectively) by controlling molecular weight of chitosan, feed-ratios, and photo-initiator concentration. Ear-shaped, hybrid scaffolds were fabricated by a stereolithographic method using a 405 nm laser. Hybrid hydrogel scaffolds of chitosan (50–190 kDa) and PEGDA (575 Da) were mixed at varying feed-ratios. Some of the cationic, amino groups of chitosan were neutralized by dialysis in acidic solution containing chitosan in excess of sodium acetate solution to inhibit quenching of newly formed photoradicals. A feed-ratio of 1:7.5 was found to be the most appropriate of the formulations considered in this study in terms of mechanical properties, cell adhesion, and printability. The biofabricated hybrid scaffold showed interconnected, homogeneous pores with a nominal pore size of 50 µm and an elastic modulus of ~400 kPa. Moreover, long-term cell viability and cell spreading was observed via actin filament staining. Printability of the biocompatible resin was confirmed by printing thresholded MR images of an ear and the feed ratio of 1:7.5 provided the most faithful reproduction of the shape. To the best of our knowledge, this is the first report of stereolithographic printing hybridizing cell-adhesive properties of chitosan with mechanical robustness of PEG in scaffolds suitable for repair of complex tissue geometries, such as those of the human ear.
Starting Page	286
Ending Page	296
Page Count	11
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	45
Issue Number	1
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2016-05-10
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Stereolithography Chitosan PEGDA Mechanical Properties Biocompatibility Aural repair 3D printing Artificial ear Biomedicine Biomedical Engineering Biological and Medical Physics, Biophysics Classical Mechanics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

3D-Printing Technologies for Craniofacial Rehabilitation, Reconstruction, and Regeneration

Stereolithography-Based 3D Printed “Pillar Plates” that Minimizes Fluid Transfers During Enzyme Linked Immunosorbent Assays

Design and Mechanical Testing of 3D Printed Hierarchical Lattices Using Biocompatible Stereolithography

3D Printed Modeling of the Mitral Valve for Catheter-Based Structural Interventions

Effect of Chemistry on Osteogenesis and Angiogenesis Towards Bone Tissue Engineering Using 3D Printed Scaffolds

3D Bioprinting for Vascularized Tissue Fabrication

Development of 3D-Printed Sulfated Chitosan Modified Bioresorbable Stents for Coronary Artery Disease

Gradient 3D Printed PLA Scaffolds on Biomedical Titanium: Mechanical Evaluation and Biocompatibility

Biocompatible 3D Printed Chitosan-Based Scaffolds Containing α-Tocopherol Showing Antioxidant and Antimicrobial Activity

Mechanical Properties, Cytocompatibility and Manufacturability of Chitosan:PEGDA Hybrid-Gel Scaffolds by Stereolithography

Similar Documents

3D-Printing Technologies for Craniofacial Rehabilitation, Reconstruction, and Regeneration

Stereolithography-Based 3D Printed “Pillar Plates” that Minimizes Fluid Transfers During Enzyme Linked Immunosorbent Assays

Design and Mechanical Testing of 3D Printed Hierarchical Lattices Using Biocompatible Stereolithography

3D Printed Modeling of the Mitral Valve for Catheter-Based Structural Interventions

Effect of Chemistry on Osteogenesis and Angiogenesis Towards Bone Tissue Engineering Using 3D Printed Scaffolds

3D Bioprinting for Vascularized Tissue Fabrication

Development of 3D-Printed Sulfated Chitosan Modified Bioresorbable Stents for Coronary Artery Disease

Gradient 3D Printed PLA Scaffolds on Biomedical Titanium: Mechanical Evaluation and Biocompatibility

Biocompatible 3D Printed Chitosan-Based Scaffolds Containing α-Tocopherol Showing Antioxidant and Antimicrobial Activity

Mechanical Properties, Cytocompatibility and Manufacturability of Chitosan:PEGDA Hybrid-Gel Scaffolds by Stereolithography