NDLI: Detecting Mitoses in Time-Lapse Images of Embryonic Epithelia Using Intensity Analysis

Content Provider	Springer Nature Link
Author	Siva, Parthipan Brodland, G. Wayne Clausi, David
Copyright Year	2009
Abstract	Although the frequency and orientation of mitoses can significantly affect the mechanics of early embryo development, these data have not been available due to a shortage of suitable automated techniques. Fluorescence imaging, though popular, requires biochemical intervention and is not always possible or desirable. Here, a new technique that takes advantage of a localized intensity change that occurs in bright field images is used to identify mitoses. The algorithm involves mapping a deformable, sub-cellular triangular mesh from one time-lapse image to the next so that corresponding regions can be identified. Triangles in the mesh that undergo darkening of a sufficient degree over a period consistent with mitosis are flagged. Mitoses are assumed to occur along the short axis of elliptical areas fit to suitably sized clusters of flagged triangles. The algorithm is less complex than previous approaches and it has strong discrimination characteristics. When applied to 15 image sets from neurulation-stage axolotl (Ambystoma mexicanum) embryos, it was able to correctly detect 86% of the manually identified mitoses, had less than 5% false positives and produced average angular errors of only 15°. The new algorithm is simpler to implement than those previously available, is substantially more accurate, and provides data that is important for understanding the mechanics of morphogenetic movements.
Starting Page	2646
Ending Page	2655
Page Count	10
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	37
Issue Number	12
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2009-09-15
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Mitosis identification Mitosis orientation Embryonic epithelia Morphogenetic movements Axolotl Biochemistry Mechanics Biophysics and Biological Physics Biomedical Engineering Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Detecting Mitoses in Time-Lapse Images of Embryonic Epithelia Using Intensity Analysis

Annals of Biomedical Engineering (Ó 2009) DOI: 10.1007/s10439-009-9790-2 Detecting Mitoses in Time-Lapse Images of Embryonic Epithelia Using Intensity Analysis (2009)

Cinemechanometry (CMM): A Method to Determine the Forces that Drive Morphogenetic Movements from Time-Lapse Images

Effect of Focal Adhesion Proteins on Endothelial Cell Adhesion, Motility and Orientation Response to Cyclic Strain

Mechanical Stress as a Regulator of Cytoskeletal Contractility and Nuclear Shape in Embryonic Epithelia

Measurement of in vivo Stress Resultants in Neurulation-stage Amphibian Embryos

Differentiation of Embryonic Stem Cells into Cardiomyocytes in a Compliant Microfluidic System

Nonlinear Stochastic System Identification of Skin Using Volterra Kernels

Detecting Biochemical Changes in the Rodent Cervix During Pregnancy Using Raman Spectroscopy

Detecting Mitoses in Time-Lapse Images of Embryonic Epithelia Using Intensity Analysis

Similar Documents

Detecting Mitoses in Time-Lapse Images of Embryonic Epithelia Using Intensity Analysis

Annals of Biomedical Engineering (Ó 2009) DOI: 10.1007/s10439-009-9790-2 Detecting Mitoses in Time-Lapse Images of Embryonic Epithelia Using Intensity Analysis (2009)

Cinemechanometry (CMM): A Method to Determine the Forces that Drive Morphogenetic Movements from Time-Lapse Images

Effect of Focal Adhesion Proteins on Endothelial Cell Adhesion, Motility and Orientation Response to Cyclic Strain

Mechanical Stress as a Regulator of Cytoskeletal Contractility and Nuclear Shape in Embryonic Epithelia

Measurement of in vivo Stress Resultants in Neurulation-stage Amphibian Embryos

Differentiation of Embryonic Stem Cells into Cardiomyocytes in a Compliant Microfluidic System

Nonlinear Stochastic System Identification of Skin Using Volterra Kernels

Detecting Biochemical Changes in the Rodent Cervix During Pregnancy Using Raman Spectroscopy

Detecting Mitoses in Time-Lapse Images of Embryonic Epithelia Using Intensity Analysis