NDLI: Boundary Stiffness Regulates Fibroblast Behavior in Collagen Gels

Content Provider	Springer Nature Link
Author	John, Jeffrey Throm Quinlan, Angela Silvestri, Chiara Billiar, Kristen
Copyright Year	2009
Abstract	Recent studies have illustrated the profound dependence of cellular behavior on the stiffness of 2D culture substrates. The goal of this study was to develop a method to alter the stiffness cells experience in a standard 3D collagen gel model without affecting the physiochemical properties of the extracellular matrix. A device was developed utilizing compliant anchors (0.048–0.64 N m−1) to tune the boundary stiffness of suspended collagen gels in between the commonly utilized free and fixed conditions (zero and infinite stiffness boundary stiffness). We demonstrate the principle of operation with finite element analyses and a wide range of experimental studies. In all cases, boundary stiffness has a strong influence on cell behavior, most notably eliciting higher basal tension and activated force (in response to KCl) and more pronounced remodeling of the collagen matrix at higher boundary stiffness levels. Measured equibiaxial forces for gels seeded with 3 million human foreskin fibroblasts range from 0.05 to 1 mN increasing monotonically with boundary stiffness. Estimated force per cell ranges from 17 to 100 nN utilizing representative volume element analysis. This device provides a valuable tool to independently study the effect of the mechanical environment of the cell in a 3D collagen matrix.
Starting Page	658
Ending Page	673
Page Count	16
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	38
Issue Number	3
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2009-12-10
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Mechanobiology Stiffness Fibroblast Myofibroblast Contraction Collagen gel Remodeling Biochemistry Mechanics Biophysics and Biological Physics Biomedical Engineering Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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