NDLI: Mechanical Interactions of Mouse Mammary Gland Cells with Collagen in a Three-Dimensional Construct

Content Provider	Springer Nature Link
Author	Lopez Garcia, M. d. C. Beebe, D. J. Crone, W. C.
Copyright Year	2010
Abstract	An effort to understand the development of breast cancer motivates the study of mammary gland cells and their interactions with the extracellular matrix. A mixture of mammary gland epithelial cells (normal murine mammary gland), collagen, and fluorescent beads was loaded into microchannels and observed via four-dimensional imaging. Collagen concentrations of 1.3, 2, and 3 mg/mL were used. The displacements of the beads were used to calculate strains in the 3D matrix. To ensure physiologically relevant materials properties for analysis, the collagen was characterized using independent tensile testing with strain rates in the range of those measured in the cell–gel constructs. 3D elastic theory for an isotropic material was employed to calculate the stress. The technique presented adds to the field of measuring cell-generated stresses by providing the capability of measuring 3D stresses locally around a single cell and using physiologically relevant materials properties for analysis. The highest strains were observed in the most compliant matrix. Additionally, the stresses fluctuated over time due to the cells’ interaction with the collagen matrix.
Starting Page	2485
Ending Page	2498
Page Count	14
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	38
Issue Number	8
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2010-04-22
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Stress Strain Collagen Young’s modulus Mammographic density Biochemistry Mechanics Biophysics and Biological Physics Biomedical Engineering Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Elastic and Viscoelastic Characterization of Mouse Oocytes Using Micropipette Indentation

Relative Contributions of Collagen and Elastin to Elasticity of the Vocal Fold Under Tension

Effect of the Structural Water on the Mechanical Properties of Collagen-like Microfibrils: A Molecular Dynamics Study

Biomechanics of Atherosclerotic Coronary Plaque: Site, Stability and In Vivo Elasticity Modeling

Quantitative Assessment of Mammary Gland Density in Rodents Using Digital Image Analysis

Using Tendon Inherent Electric Properties to Consistently Track Induced Mechanical Strain

Dielectrophoresis-Mediated Electrodeformation as a Means of Determining Individual Platelet Stiffness

A Microplate Compression Method for Elastic Modulus Measurement of Soft and Viscoelastic Collagen Microspheres

Mechanical, Compositional, and Structural Properties of the Post-natal Mouse Achilles Tendon

Mechanical Interactions of Mouse Mammary Gland Cells with Collagen in a Three-Dimensional Construct

Similar Documents

Elastic and Viscoelastic Characterization of Mouse Oocytes Using Micropipette Indentation

Relative Contributions of Collagen and Elastin to Elasticity of the Vocal Fold Under Tension

Effect of the Structural Water on the Mechanical Properties of Collagen-like Microfibrils: A Molecular Dynamics Study

Biomechanics of Atherosclerotic Coronary Plaque: Site, Stability and In Vivo Elasticity Modeling

Quantitative Assessment of Mammary Gland Density in Rodents Using Digital Image Analysis

Using Tendon Inherent Electric Properties to Consistently Track Induced Mechanical Strain

Dielectrophoresis-Mediated Electrodeformation as a Means of Determining Individual Platelet Stiffness

A Microplate Compression Method for Elastic Modulus Measurement of Soft and Viscoelastic Collagen Microspheres

Mechanical, Compositional, and Structural Properties of the Post-natal Mouse Achilles Tendon

Mechanical Interactions of Mouse Mammary Gland Cells with Collagen in a Three-Dimensional Construct