NDLI: Development of Vibrational Culture Model Mimicking Vocal Fold Tissues

Content Provider	Springer Nature Link
Author	Kim, Dongjoo Lim, Jae Yol Kwon, Soonjo
Copyright Year	2016
Abstract	The vocal folds (VFs) are connective tissues with complex matrix structures that provide the required mechanical properties for voice generation. VF injury leads to changes in tissue structure and properties, resulting in reduced voice quality. However, injury-induced biochemical changes and repair in scarred VF tissues have not been well characterized to date. To treat scarred VFs, it is essential to understand how physiological characteristics of VFs tissue change in response to external perturbation. In this study, we designed a simple vibrational culture model to mimic vibratory microenvironments observed in vivo. This model consists of a flexible culture plate, three linear actuators, a stereo splitter, and a function generator. Human vocal fold fibroblast (hVFF) monolayers were established on the flexible membrane, to which normal phonatory vibrations were delivered from linear actuators and a function generator. The hVFF monolayers were exposed to the vibrational stresses at a frequency of 205 Hz for 2, 6, and 10 h with maximum displacement of 47.1 μm, followed by a 6 h rest. We then observed the changes in cell morphology, cell viability, and gene expression related to extracellular matrix components. In our dynamic culture device mimicking normal phonatory frequencies, cell proliferation increased and expression of hyaluronic acid synthase 2 was downregulated in response to vibrational stresses. The results presented herein will be useful for evaluating cellular responses following VF injuries in the presence or absence of vibrational stresses.
Starting Page	3136
Ending Page	3143
Page Count	8
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	44
Issue Number	10
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2016-03-07
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Vocal folds Dynamic culture model Phonatory vibration Frequency ECM proteins Biomedicine general Biomedical Engineering Biological and Medical Physics, Biophysics Classical Mechanics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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