NDLI: Disturbed Cyclical Stretch of Endothelial Cells Promotes Nuclear Expression of the Pro-Atherogenic Transcription Factor NF-κB

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Disturbed Cyclical Stretch of Endothelial Cells Promotes Nuclear Expression of the Pro-Atherogenic Transcription Factor NF-κB

Content Provider	Springer Nature Link
Author	Pedrigi, Ryan M. Papadimitriou, Konstantis I. Kondiboyina, Avinash Sidhu, Sukhjinder Chau, James Patel, Miten B. Baeriswyl, Daniel C. Drakakis, Emmanuel M. Krams, Rob
Copyright Year	2016
Abstract	Exposure of endothelial cells to low and multidirectional blood flow is known to promote a pro-atherogenic phenotype. The mechanics of the vessel wall is another important mechano-stimulus within the endothelial cell environment, but no study has examined whether changes in the magnitude and direction of cell stretch can be pro-atherogenic. Herein, we developed a custom cell stretching device to replicate the in vivo stretch environment of the endothelial cell and examined whether low and multidirectional stretch promote nuclear translocation of NF-κB. A fluid–structure interaction model of the device demonstrated a nearly uniform strain within the region of cell attachment and a negligible magnitude of shear stress due to cyclical stretching of the cells in media. Compared to normal cyclical stretch, a low magnitude of cyclical stretch or no stretch caused increased expression of nuclear NF-κB (p = 0.09 and p < 0.001, respectively). Multidirectional stretch also promoted significant nuclear NF-κB expression, comparable to the no stretch condition, which was statistically higher than the low (p < 0.001) and normal (p < 0.001) stretch conditions. This is the first study to show that stretch conditions analogous to atherogenic blood flow profiles can similarly promote a pro-atherogenic endothelial cell phenotype, which supports a role for disturbed vessel wall mechanics as a pathological cell stimulus in the development of advanced atherosclerotic plaques.
Starting Page	898
Ending Page	909
Page Count	12
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	45
Issue Number	4
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2016-10-27
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Atherosclerosis Mechanobiology Biomechanics Strain Shear stress Fluid–structure interaction Advanced plaques Thin cap fibroatheroma Nuclear factor kappa b Biomedicine Biomedical Engineering Biological and Medical Physics, Biophysics Classical Mechanics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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