NDLI: An improved method of photometric determination of cyclodextrin glucanotransferase activity

Content Provider	Springer Nature Link
Author	Usav, N. G. Gil’vava, E. A. Elizar’ev, P. A. Prutsakova, E. A. Melent’ev, A. I.
Copyright Year	2007
Abstract	The method of spectrophotometric determination of cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) activity with the use of phenolphthalein as a colored reagent has been improved. This technique includes an enzymatic reaction at 40°C for 60 min in 2% starch, with subsequent supplementation of the reaction mixture (0.5 ml) with the phenolphthalein reagent (3.0 ml) prepared in 0.1 M potassium carbonate buffer (pH 11.0) according to a special procedure, and measurement of the optical density of the obtained mixture at 553 nm. The activity was calculated using the exponential growth equation that connects a drop in the optical density and the degree of dilution of the enzyme. The described technique is suitable for working in a sufficiently broad range of specific activity of β-CGTase and does not require precise adjustment of the degree of dilution of solutions analyzed.
Starting Page	105
Ending Page	110
Page Count	6
File Format	PDF
ISSN	00036838
Journal	Applied Biochemistry and Microbiology
Volume Number	43
Issue Number	1
e-ISSN	16083024
Language	English
Publisher	Nauka/Interperiodica
Publisher Date	2007-01-01
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Medical Microbiology Biochemistry Microbiology
Content Type	Text
Resource Type	Article
Subject	Biochemistry Applied Microbiology and Biotechnology

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An improved method of photometric determination of cyclodextrin glucanotransferase activity