NDLI: Bioengineering of symbiotic systems: Creation of new associative symbiosis with the use of lectins on the example of tobacco and oil seed rape

Content Provider	Springer Nature Link
Author	Vershinina, Z. R. Baimiev, An. Kh. Blagova, D. K. Knyazev, A. V. Baimiev, Al. Kh. Chemeris, A. V.
Copyright Year	2011
Abstract	Hairy roots in tobacco and oil seed rape transgenic on lectin gene were obtained with the use of a wild strain of Agrobacterium rhizogenes 15834 transformed with pCAMBIA1305.1 plasmid containing the full-size lectin gene (psl) from the Pisum sativum. Influence of expression of lectin gene on colonization of transgenic roots with symbiont of pea (Rhizobium leguminosarum) was investigated. The number of adhered bacteria onto the roots transformed with lectin gene was 14-fold and 37-fold higher in comparison with the control; this confirms the interaction of R. leguminosarum with pea lectin at the surface of the transformed roots of tobacco and oil seed rape. The developed experimental approach, based on the simulation of recognition processes and early symbiotic interactions with lectins of pea plants, may, in perspective, be used for obtaining stable associations of economically valuable, nonsymbiotrophic plant species with rhizobia.
Starting Page	304
Ending Page	310
Page Count	7
File Format	PDF
ISSN	00036838
Journal	Applied Biochemistry and Microbiology
Volume Number	47
Issue Number	3
e-ISSN	16083024
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2011-05-06
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microbiology Biochemistry Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Biochemistry Applied Microbiology and Biotechnology

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