NDLI: Aerobic methylobacteria as the basis for a biosensor for dichloromethane detection

Content Provider	Springer Nature Link
Author	Plekhava, Yu. V. Firsova, Yu. E. Doronina, N. V. Trotsenko, Yu. A. Reshetilov, A. N.
Copyright Year	2013
Abstract	Cells of dichloromethane (DChM) bacteria-destructors were immobilized by sorption on different types of membranes, which were fixed on the measuring surface of a pH-sensitive field transistor. The presence of DChM in the medium (0.6–8.8 mM) led to a change in the transistor’s output signal, which was determined by the appearance of H+ ions in the medium due to DChM utilization by methylobateria. Among four strains of methylobacteria—Methylobacterium dichloromethanicum DM4, Methylobacterium extorquens DM17, Methylopila helvetica DM6, and Ancylobacter dichloromethanicus DM16—the highest and most stable activity toward DChM degradation was observed in the strain M. dichloromethanicum DM4. Among 11 types of membranes for cell immobilization, Millipore nitrocellulose membranes and chromatographic fiber paper GF/A, which allow one to obtain stable biosensor signals for 2 weeks without a bioreceptor change, were chosen as optimal carriers.
Starting Page	188
Ending Page	193
Page Count	6
File Format	PDF
ISSN	00036838
Journal	Applied Biochemistry and Microbiology
Volume Number	49
Issue Number	2
e-ISSN	16083024
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2013-02-26
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biochemistry Microbiology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Biochemistry Applied Microbiology and Biotechnology

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Adaptation of aerobic methylobacteria to dichloromethane degradation

Aerobic methylobacteria as promising objects of modern biotechnology (Review)

Effects of DNA-damaging Agents on Aerobic Methylobacteria Capable and Incapable of Utilizing Dichloromethane

Functionality of the xoxF gene in Methylobacterium dichloromethanicum DM4

Functionality of Metdi5511gene in Methylobacterium dichloromethanicum DM4

Analysis of the 3′-region of the dcmA gene of dichloromethane dehalogenase of Methylobacterium dichloromethanicum DM4

Functional activity of the modA $_{ 2 }$ gene in Methylobacterium dichloromethanicum DM4

Physiological and Biochemical Analysis of the Transformants of Aerobic Methylobacteria Expressing the dcmA Gene of Dichloromethane Dehalogenase

Phosphate-solubilizing activity of aerobic methylobacteria

Aerobic methylobacteria as the basis for a biosensor for dichloromethane detection

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