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| Content Provider | Springer Nature Link |
|---|---|
| Author | Feng, H. W. Zhi, Y. E. Sun, Y. J. Xu, L. R. Wang, L. M. Zhan, X. J. Zhou, P. |
| Copyright Year | 2016 |
| Abstract | Streptomyces griseorubens JSD-1 is novel isolate utilizing cellulose as a sole carbon source for growth, and cellulytic genes related to this proceßs were expected to be crucial. To investigate the molecular mechanism of cellulose metabolism, bacterial genome sequencing was carried out. As a result, its draft genome map was obtained which has been deposited at GenBank. ß-1,4-glucosidase (Glu) was acquired following with characterization of its cellular localization and expression profiles. Glu from S. griseorubens JSD-1 was suggested to be an intracellular enzyme which was different from those obtained from similar species through the combined analysis of multiple sequences alignment and putative 3D structure modeling. Expression of recombinant enzyme was optimized in heterogeneous hosts for the further determination of its optimum pH and temperature as well as the residual activity in the presence of metal ions and inhibitors. Novel Glu expected to have promising potential in the degradation of cellulosic materials. |
| Starting Page | 371 |
| Ending Page | 377 |
| Page Count | 7 |
| File Format | |
| ISSN | 00036838 |
| Journal | Applied Biochemistry and Microbiology |
| Volume Number | 52 |
| Issue Number | 4 |
| e-ISSN | 16083024 |
| Language | English |
| Publisher | Pleiades Publishing |
| Publisher Date | 2016-07-27 |
| Publisher Place | Moscow |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Streptomyces griseorubens cellulose β-1,4-glucosidase enzymatic activity Biochemistry Microbiology Medical Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Applied Microbiology and Biotechnology |
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