NDLI: Screening of taxol-producing endophytic fungi from Taxus chinensis var. mairei

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Screening of taxol-producing endophytic fungi from Taxus chinensis var. mairei

Content Provider	Springer Nature Link
Author	Zhou, X. Wang, Z. Jiang, K. Wei, Y. Lin, J. Sun, X. Tang, K.
Copyright Year	2007
Abstract	A total of 38 endophytic fungus strains were isolated from Taxus chinensis var. mairei by the aseptic technique. Genomic DNA was extracted from isolated endophytic fungi and subjected to polymerase chain reaction (PCR) analysis for the presence of the Taxus taxadiene synthase (TS) gene, a rate-limiting enzyme gene in the taxol biosynthetic pathway. Twelve out of 38 isolated endophytic fungus strains showed PCR positive for the ts gene. Subsequently, taxol and its related compounds were extracted from culture filtrates and mycelia of the PCR positive strains, separated by column chromatography, and analyzed by High Performance Liquid Chromatography and Mass Spectrum. The analysis result showed that 3 strains could produce taxol and its related compounds at the detectible level. This study indicates that molecular detection of the ts gene is an efficient method for primary screening of taxol or its related compound-producing endophytic fungi, which can improve prominently screening efficiency.
Starting Page	439
Ending Page	443
Page Count	5
File Format	PDF
ISSN	00036838
Journal	Applied Biochemistry and Microbiology
Volume Number	43
Issue Number	4
e-ISSN	16083024
Language	English
Publisher	Nauka/Interperiodica
Publisher Date	2007-01-01
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biochemistry Microbiology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Biochemistry Applied Microbiology and Biotechnology

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