NDLI: Resistance of Penicillium piceum F-648 to Hydrogen Peroxide under Short-Term and Prolonged Oxidative Stress

Content Provider	Springer Nature Link
Author	Pavlovskaya, Zh. I. Mikhailova, R. V. Moroz, I. V. Eremin, A. N.
Copyright Year	2003
Abstract	Resistance of Penicillium piceumF-648 to hydrogen peroxide under short-term and prolonged oxidative stress was studied. An increase in the activity of intracellular catalase in fungal cells after short-term exposure to hydrogen peroxide was shown. Activation of fungal cells induced by H2O2 depends on the H2O2 concentration, time of exposure, and growth phase of the fungus. Variants of P. piceum F-648 that produced two forms of extracellular catalase with different catalytic properties were obtained due to prolonged adaptation to H2O2. Catalase with low affinity for substrate was produced predominantly by the parent culture and variant 3; however, a high substrate affinity of catalase was observed in variant 5. Variant 5 of P. piceum F-648 displayed a high catalytic activity and operational stability of catalase in the presence of phosphate ions and a concentration of substrate less than 30 mM at pH more than 7.
Starting Page	24
Ending Page	29
Page Count	6
File Format	PDF
ISSN	00036838
Journal	Applied Biochemistry and Microbiology
Volume Number	39
Issue Number	1
e-ISSN	16083024
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2003-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Medical Microbiology Biochemistry Microbiology
Content Type	Text
Resource Type	Article
Subject	Biochemistry Applied Microbiology and Biotechnology

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Kinetic Characterization of Extracellular Catalases fromPenicillium piceum F-648 and Its Hydrogen Peroxide-Adapted Variants

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