NDLI: Blister pouches for effective reagent storage on microfluidic chips for blood cell counting

Content Provider	Springer Nature Link
Author	Smith, Suzanne Sewart, René Becker, Holger Roux, Pieter Land, Kevin
Copyright Year	2016
Abstract	We present the analysis of blister pouches for reagent storage and release into microfluidic devices towards point-of-care blood cell counting applications. Blister pouches provide an effective reagent storage mechanism and can be mounted onto microfluidic cartridges directly. Reagents can be released from blister pouches through automated or manual compression and consequent rupturing of the pouches. A microfluidic device for metering of blister pouch contents was developed and investigated as part of this work, as precise volumes of reagents are often required when performing reactions, and particularly for blood cell counting applications which are the focus of this study. The metering device shows high accuracy and repeatability with an error of 1.93% and standard deviation of 3.1% across 30 test results. This work also investigates important blister pouch characteristics for three different types of blister pouch foil materials, including forces required to burst the blister, as well as shelf life and reagent compatibility of the blisters. Typical forces required are in the range of 25–35 N depending on the blister foil material used. Blister shelf life can be greatly affected by the reagent being stored, and thus, the blister foil material choice is crucial. This work provides a clear understanding of the implementation required to ensure that the blister pouches can be effectively used on microfluidic chips, with an example application area being point-of-care diagnostics.
Starting Page	1
Ending Page	14
Page Count	14
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	20
Issue Number	12
e-ISSN	16134990
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-11-23
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Blister pouch Microfluidics Reagent storage Reagent metering Point-of-care diagnostics Engineering Fluid Dynamics Biomedical Engineering Analytical Chemistry Nanotechnology and Microengineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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