NDLI: Development of sorting, aligning, and orienting motile sperm using microfluidic device operated by hydrostatic pressure

Content Provider	Springer Nature Link
Author	Seo, Duck bong Agca, Yuksel Feng, Z. C. Critser, John K.
Copyright Year	2007
Abstract	In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are the most commonly used assisted reproductive technologies to overcome male infertility problems. One of the obstacles of IVF and ICSI procedures is separating motile sperm from non-motile sperm to select the most competent sperm population from any given sperm sample. In addition, orientation and separation of the head from the tail is another obstacle for ICSI. Using the self-movement of sperm against flow direction, motile and non-motile sperm can be separated with an inexpensive polymeric microfluidic system. In this paper, we describe the development of a microfluidic system obtained through low-cost fabrication processes. We report experimental results of sperm sorting using hydrostatic pressure of three different species: bull, mouse, and human. The movement of cells in these channels was observed under a microscope and recorded with a digital camera. It is shown that the hydrostatic pressure and self-movement of motile sperm can be used to solve separating, aligning and orienting sperm in the microchannel.
Starting Page	561
Ending Page	570
Page Count	10
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	3
Issue Number	5
e-ISSN	16134990
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-01-10
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microfluidics Motile-sperm sorting Intracytoplasmic sperm injection Engineering Thermodynamics, Transport Phenomena Mechanics, Fluids, Thermodynamics Nanotechnology Polymer Sciences Medical Microbiology Engineering Fluid Dynamics
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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