NDLI: A developed competitive immunoassay based on impedance measurements for methamphetamine detection

Content Provider	Springer Nature Link
Author	Yeh, Chia Hsien Wang, Wei Ting Shen, Pi Lan Lin, Yu Cheng
Copyright Year	2012
Abstract	In this study, an electro-microchip was successfully used to detect the impedance signals of various methamphetamine (MET) concentrations based on the developed competitive immunoassay method. MET is a commonly used drug often abused by drug addicts and can cause irregular behavior; therefore, MET concentration detection is important for quantitative analysis. In this study, gold nanoparticles (AuNPs) were introduced into an electro-microchip through the specific binding of antibodies, thus constructing a bridge between two electrodes and allowing electrons to move between them. The decreasing impedance value can be easily measured with a commercial LCR meter. According to the collected measurements, a significant difference was observed in impedance signals after 13 min when MET concentrations were reduced. Additionally, a clear, distinguished impedance (a steep slope for both impedance and MET concentration) in the frequency effect (100 Hz–1 MHz) was observed at 100 Hz. When the concentration of the anti-MET antibody–colloidal gold conjugates was diluted 100×, the detectable limit for MET concentration was 1 ng/mL with 0.5 μg/mL of BSA–MET conjugate. Therefore, the developed electro-microchip is advantageous because it is effective with small sample volumes (30 μL), is a form of rapid quantitative measurement, and works with smaller detectable concentrations than other existing commercial detection products which have the best limit of detection of 100 ng/mL.
Starting Page	319
Ending Page	329
Page Count	11
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	13
Issue Number	2
e-ISSN	16134990
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-03-21
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	An electro-microchip Methamphetamine Developed competitive immunoassay Detectable limit Nanotechnology and Microengineering Analytical Chemistry Engineering Fluid Dynamics Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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