NDLI: Development of a micro-scale perfusion 3D cell culture biochip with an incorporated electrical impedance measurement scheme for the quantification of cell number in a 3D cell culture construct

Content Provider	Springer Nature Link
Author	Lei, Kin Fong Wu, Min Hsien Liao, Pei You Chen, Yan Ming Pan, Tung Ming
Copyright Year	2011
Abstract	This article reports a perfusion 3D cell culture biochip with an incorporated electrical impedance measurement-based scheme for the quantification of cell number in the 3D cell culture construct. The biochip consists of culture chamber and fluidic channel for perfusion 3D cell culture followed by assembling the electrodes for impedance measurement. In this study, breast cancer cell line culture was performed using the proposed biochip. The perfusion 3D cell culture model was compared with a conventional static cell culture model in terms of environmental pH and metabolic activities of cells. Results revealed that the real value of the proposed biochip has its ability to maintain a stable and thus quantifiable culture condition. In addition, the metabolic activities of cells in the two culture models compared showed statistically different. It is concluded that the choice of a cell culture format can influence assay results. Moreover, after performing the 3D cell culture, electrical impedance measurement was conducted for the quantification of cell number in the 3D cell culture construct. The results indicated that the change in impedance was directly proportional to the cell number from 104 to 107 cells ml−1 in the culture construct. As a whole, the proposed biochip not only can provide a stable, well-defined, and biologically meaningful culture environment, but also integrates the work of cell number quantification in a simple, economy, and time efficient manner. All of these traits are particularly useful for high-precision and high-throughput 3D cell culture-based assays.
Starting Page	117
Ending Page	125
Page Count	9
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	12
Issue Number	1-4
e-ISSN	16134990
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-07-24
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biochip Electrical impedance Cell culture Perfusion culture Cell number Industrial Chemistry/Chemical Engineering Biomedical Engineering Engineering Fluid Dynamics
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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