NDLI: Paper-based immunoaffinity devices for accessible isolation and characterization of extracellular vesicles

Content Provider	Springer Nature Link
Author	Chen, Chihchen Lin, Bo Ren Wang, Hsi Kai Fan, Shu Ting Hsu, Min Yen Cheng, Chao Min
Copyright Year	2014
Abstract	We present a paper-based immunoaffinity platform for separating subsets of extracellular vesicles (EVs). This method is inexpensive, robust, easy-to-use (easy-to-prepare), and compatible with downstream analyses, such as scanning electron microscopy (SEM), enzyme-linked immunosorbent assays (ELISA), and transcriptome analysis. EVs are small membranous vesicles shed from both healthy and diseased cells and contain nucleic acid and protein cargo. EVs have been increasingly recognized as a means of cell–cell communication and hold a great potential for clinical applications. However, current protocols for isolation of EVs are often lengthy, cumbersome and require expensive equipment. Here, we have isolated EVs from small volumes of both human serum and aqueous humor samples using paper-based immunoaffinity devices. Captured EVs were analyzed morphologically using SEM and appeared statistically different in size (p value < 2.4 × 10−22) and circularity (p value < 3.6 × 10−9) between subsets of EVs bearing different surface markers. Assessing the amount of EVs captured using paper-based ELISA using antibodies conjugated to horseradish peroxidase to produce a colorimetric readout was accomplished within 10 min. RNAs contained in EVs can be extracted to provide information for disease management. These paper-based immunoaffinity devices, we believe, open opportunities for a wide range of applications in both basic biology and clinical medicine.
Starting Page	849
Ending Page	856
Page Count	8
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	16
Issue Number	5
e-ISSN	16134990
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-02-08
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Extracellular vesicles Exosomes Paper microfluidics Aqueous humor Chemical conjugation Engineering Fluid Dynamics Biomedical Engineering Analytical Chemistry Nanotechnology and Microengineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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