NDLI: Experimental studies and modeling of α-amylase aqueous two-phase extraction within a microfluidic device

Content Provider	Springer Nature Link
Author	vak, Uroš Lakner, Mitja Plazl, Igor Žnidaršič Plazl, Polona
Copyright Year	2015
Abstract	Polyethylene glycol (PEG)/phosphate aqueous two-phase system was used for continuous extraction of α-amylase within glass microfluidic chips with Y- and Ψ-branched inflow and outflow channels. By establishing stable parallel flows with interfaces at the position of inlet/outlet channels junctures, the separation of phases at the exit of microfluidic devices was attained. The use of a Ψ-branched microchannel assisting creation of two interfacial layers for α-amylase transfer to the PEG-rich phase enabled to reach the equilibrium stage in <10 s. On the contrary, the equilibrium was not achieved within a single pass through the Y-branched microchannel system where the efficiency was 1.8 times lower than in the Ψ-branched counterpart. However, both systems outperformed batch extraction with the same solvent system regarding time needed for achieving steady state by two orders of magnitude. A 3D mathematical model for steady-state conditions was developed, comprising velocity profile within the channel, mass transport by convection in the flow direction and diffusion in all spatial directions, as well as equilibrium concentrations at the interfacial areas, which enables accurate process description and further process optimization and development by the numbering-up concept.
Starting Page	75
Ending Page	83
Page Count	9
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	19
Issue Number	1
e-ISSN	16134990
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-02-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Aqueous two-phase system Microchannel Extraction α-amylase Model Parallel flow Engineering Fluid Dynamics Biomedical Engineering Analytical Chemistry Nanotechnology and Microengineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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