NDLI: Centrifugo-magnetophoretic particle separation

Content Provider	Springer Nature Link
Author	Kirby, Daniel Siegrist, Jonathan Kijanka, Gregor Zavattoni, Laëtitia Sheils, Orla O’Leary, John Burger, Robert Ducrée, Jens
Copyright Year	2012
Abstract	There has been a recent surge of research output on magnetophoretic lab-on-a-chip systems due to their prospective use in a range of applications in the life sciences and clinical diagnostics. Manifold applications for batch-mode or continuous-flow magnetophoretic separations of cells, proteins, and nucleic acids are found in bioanalytics, cell biology, and clinical diagnostics. To ensure stable hydrodynamic conditions and thus reproducible separation, state-of-the-art magnetophoretic lab-on-a-chip systems have been based on pressure-driven flow (Gijs in Microfluid Nanofluid 1:22–40, 2004; Pamme and Manz in Anal Chem 76:7250–7256, 2004; Pamme in Lab Chip 7:1644–1659, 2007; Karle et al. in Lab Chip 10:3284–3290, 2010), which involves rather bulky and costly instrumentation. In a flow-based system, suspended particles are following the liquid phase as a result of the Stokes drag, thus being fully exposed to divergent flow lines around obstacles and pump-induced pressure fluctuations. To eventually achieve more stable hydrodynamic conditions, improved control of magnetic particles, a more compact instrumentation footprint, and integration of high-performance upstream sample preparation, this work introduces a novel two-dimensional particle separation principle by combining magnetic deflection with centrifugal sedimentation in a stopped-flow mode (i.e., mere particle sedimentation). The experimental parameters governing our centrifugo-magnetophoretic system are the strength and orientation of the co-rotating magnetic field, the rotationally induced centrifugal field, and the size-dependent Stokes drag of the various particles with respect to the (residual) liquid phase. In this work, the following set of basic functional modes is demonstrated as proof-of-concept: separation of magnetic from non-magnetic particles, routing of magnetic particles based on control of the spin speed, and size separation of various magnetic particles. Finally, a biomimetic application involving the separation of particles representing healthy cells from a very small concentration of magnetic particles of a similar size, mass and magnetization as a immuno-magnetically tagged target cell, for instance mimicking a circulating tumor cell.
Starting Page	899
Ending Page	908
Page Count	10
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	13
Issue Number	6
e-ISSN	16134990
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-07-08
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Centrifugal Microfluidic Magnetophoresis Separation Particles Analytical Chemistry Engineering Fluid Dynamics Nanotechnology and Microengineering Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Inertially focused diamagnetic particle separation in ferrofluids

A magnetophoresis-based microfluidic detection platform under a static-fluid environment

Fabrication and integration of microscale permanent magnets for particle separation in microfluidics

Microfluidic counterflow centrifugal elutriation system for sedimentation-based cell separation

Computational and performance analysis of a continuous magnetophoretic bioseparation chip with alternating magnetic fields

Particle/cell separation on microfluidic platforms based on centrifugation effect: a review

Combining positive and negative magnetophoreses to separate particles of different magnetic properties

Microfluidic separation of magnetic particles with soft magnetic microstructures

Particle separation and sorting in microfluidic devices: a review

Centrifugo-magnetophoretic particle separation

Similar Documents

Inertially focused diamagnetic particle separation in ferrofluids

A magnetophoresis-based microfluidic detection platform under a static-fluid environment

Fabrication and integration of microscale permanent magnets for particle separation in microfluidics

Microfluidic counterflow centrifugal elutriation system for sedimentation-based cell separation

Computational and performance analysis of a continuous magnetophoretic bioseparation chip with alternating magnetic fields

Particle/cell separation on microfluidic platforms based on centrifugation effect: a review

Combining positive and negative magnetophoreses to separate particles of different magnetic properties

Microfluidic separation of magnetic particles with soft magnetic microstructures

Particle separation and sorting in microfluidic devices: a review

Centrifugo-magnetophoretic particle separation