NDLI: Direct pattern formation of bacterial cells using micro-droplets generated by electrohydrodynamic forces

Content Provider	Springer Nature Link
Author	Kim, Ju Han Lee, Dae Young Hwang, Jungho Jung, Hyo Il
Copyright Year	2009
Abstract	Bacterial cells were patterned efficiently on a nutrient-coated membrane in a direct, rapid, and cost-effective manner using electro-hydrodynamically generated micro-droplets. By varying the viscosity and electrical conductivity of the bacterial printing solution, uniform droplets were obtained. Escherichia coli cells were directly printed onto a membrane filter, followed by overnight incubation on an agar plate which created line patterns of bacterial colonies with a width of approximately 160 μm. Optimization of the concentration of the chemical components [i.e. the ethylene glycol (EG), phosphate buffer (PB), and sodium chloride (NaCl)] in the processing bacterial solution allowed successful growth and patterning of the cells. The optimal conditions to achieve the most effective cell growth and patterning on these printed surfaces were an EG concentration of 40 vol% and a concentration as low as 10 mM of PB or NaCl in the printing solution. Cells passing through the electric nozzle during the printing process remained viable.
Starting Page	829
Ending Page	839
Page Count	11
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	7
Issue Number	6
e-ISSN	16134990
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-04-16
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Electrohydrodynamic printing Droplets Bacterial cells Micropatterning Cone-jet Biomedical Engineering Engineering Fluid Dynamics
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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