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| Content Provider | Springer Nature Link |
|---|---|
| Author | Kolodziej, K. Theissinger, K. Brün, J. Schulz, H. K. Schulz, R. |
| Copyright Year | 2011 |
| Abstract | In the context of developing a noninvasive, practicable method for population size estimation in wild boar, we present a stepwise procedure to reduce the number of required microsatellite markers for individual genotyping. Step1: an initial marker set of 12 microsatellite loci was tested for species specificity with nontarget DNA and resulted in an exclusion of two markers. Step 2: a variability test regarding heterozygosity and deviations from Hardy–Weinberg equilibrium led to the rejection of two further markers. Step 3: the remaining eight markers were tested for transferability across populations with three separate wild boar sample sets. Step 4: on the basis of probability of identity values, a reduction from eight to five markers was possible. Step 5: a novel test using tissue samples from female wild boars and their embryos provided evidence that four variable microsatellite markers and one sex marker are sufficient for individual identification of close relatives. Step 6: feces samples were finally used to estimate PCR (PS) and genotyping success (GS). In conclusion, we recommend a specific four-marker combination with both PS and GS >50% for a reliable individual identification in noninvasive population size estimation of wild boar. |
| Starting Page | 621 |
| Ending Page | 628 |
| Page Count | 8 |
| File Format | |
| ISSN | 16124642 |
| Journal | European Journal of Wildlife Research |
| Volume Number | 58 |
| Issue Number | 3 |
| e-ISSN | 14390574 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2011-11-12 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Noninvasive sampling Feces Wildlife forensics Population size estimation Marker specificity test Reliable genotypes Animal Ecology Zoology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ecology, Evolution, Behavior and Systematics Management, Monitoring, Policy and Law Nature and Landscape Conservation |
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