NDLI: Capacitation status of frozen–thawed spermatozoa from wild ruminant species

Content Provider	Springer Nature Link
Author	Mejia, M. Medra, A. Gonzalez Rebeles, C. Mejia, O.
Copyright Year	2008
Abstract	Semen from Barbary sheep (Ammotragus lervia), Bighorn sheep (Ovis canadensis), Mouflon sheep (Ovis musimon), Fallow deer (Dama dama), collected by electroejaculation, and semen from Wildebeest antelope (Connochaetes taurinus), collected post mortem, were frozen using a standardized technique and a commercial freezing medium (Triladyl). Sperm quality was assessed by measuring their capacitation status (chlortetracycline assay) in addition to classic assessment: motility, viability (plasma membrane integrity), and acrosome integrity. Sperm cryosurvival measured in terms of recovery (from the initial, pre-freeze values) of motility, viability, and acrosome integrity was relatively high in most species. However, proportion of premature-capacitated spermatozoa (B + AR patterns) increased many times in relation to that of fresh semen: from 8% to 78% in Barbary sheep; from 27% to 61% in Bighorn sheep; and from 12% to 68% in Mouflon sheep. The use of a standardized technique for freezing of spermatozoa from wild ruminant species produced, in most of the cases, acceptable results. This approach may be extensively used to carry out sperm cryopreservation in field conditions. Chlortetracycline assay allowed identifying the same fluorescent patterns observed in spermatozoa from domestic animals and produced additional information on sperm cryosurvival. That is, it revealed those cells that survive freeze–thawing and are potentially fertile: non-capacitated (F pattern) and capacitated acrosome-intact spermatozoa (B pattern).
Starting Page	1
Ending Page	6
Page Count	6
File Format	PDF
ISSN	16124642
Journal	European Journal of Wildlife Research
Volume Number	55
Issue Number	1
e-ISSN	14390574
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-07-17
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Semen Cryopreservation Wild sheep Antelope Deer Animal Ecology Zoology
Content Type	Text
Resource Type	Article
Subject	Ecology, Evolution, Behavior and Systematics Management, Monitoring, Policy and Law Nature and Landscape Conservation

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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