NDLI: Obtaining high-quality DNA from elusive small mammals using low-tech hair snares

Content Provider	Springer Nature Link
Author	Henry, Philippe Russello, Michael A.
Copyright Year	2010
Abstract	Noninvasive sampling approaches are becoming increasingly important for enabling genetic studies of wildlife populations. While a number of methods have been described to noninvasively sample hair from carnivores and medium-sized mammals, they have largely remained untested in elusive small mammals. Here we describe a novel and inexpensive noninvasive hair snare targeted at an elusive small mammal, the American pika (Ochotona princeps). We explore the quality of the sample by assessing PCR amplification success of mitochondrial and nuclear DNA fragments across four commercially available DNA isolation kits and two different quantities of hair in a factorial design. Additionally, we determined the sex of the individual samples using PCR–RFLP of ZFX/ZFY loci. We found that our snare is effective in obtaining hair that yield DNA of sufficient quality and quantity to successfully amplify a range of mitochondrial and nuclear fragment sizes. Specifically, we found the greatest success in amplifying mitochondrial DNA, nuclear microsatellites and ZFX/ZFY loci using at least 25 hairs as starting material and the DNA IQ™ system. The hair snares thus provide a cost-effective and minimally intrusive approach to sample elusive or rare small mammals. We anticipate that this approach will be useful to obtain samples for molecular studies of the ecology, evolution and conservation of small, elusive mammals.
Starting Page	429
Ending Page	435
Page Count	7
File Format	PDF
ISSN	16124642
Journal	European Journal of Wildlife Research
Volume Number	57
Issue Number	3
e-ISSN	14390574
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-10-05
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microsatellites Mitochondrial DNA Noninvasive genetic sampling Molecular sexing Small mammals Ochotona princeps Animal Ecology Zoology
Content Type	Text
Resource Type	Article
Subject	Ecology, Evolution, Behavior and Systematics Management, Monitoring, Policy and Law Nature and Landscape Conservation

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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