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| Content Provider | Springer Nature Link |
|---|---|
| Author | Lutz, Walburga Junghans, Doris Schmitz, Dagmar Müller, T. |
| Copyright Year | 2003 |
| Abstract | In a long-term study, a total of 1,112 European wild boar (Sus scrofa) shot in the southern part of North Rhine Westphalia between 1993 and 2000 were investigated for the infection with pseudorabies virus (PRV). Of 929 sera tested during the 7 year observation period -92 (9.4%) had PRV-specific antibodies with an overall seroprevalence in the south west of North Rhine Westphalia of 11.4%. A total of 473 tissues and 516 mucosal swabs collected between 1996 and 2000 from wild boar originating from those districts were tested for the presence of PRV using virus isolation in cell culture. In 2000, 4 viruses were isolated from the tonsils of piglets and juvenile wild boar shot in the district of Bonn, which were identified as type Ip PRV. When tonsils and lungs were additionally tested in a glycoprotein D-specific PCR, 14 (4.0%) and 2 (1.6%) were positive. Virus isolation and PCR results suggest that PRV might be mainly transmitted by wild boar piglets. The results also indicate that PRV-infections have been endemic in wild boar populations in the south west of North Rhine Westphalia for several years. Based on present knowledge, the area covered by this study appears to be part of a larger, coherent endemic area comprising wild boar populations in neighbouring regions of western Germany. Recently, PRV was eradicated in the domestic pigs in North Rhine Westphalia resulting in a recognition as an officially Aujesky's disease free region within the EU. As in other regions, so far, PRV infections in the wild boar population appear not to affect the domestic pig population.In einer Langzeitstudie wurden zwischen 1993 und 2000 insgesamt 1112 Stück Schwarzwild (Sus scrofa) aus dem südlichen Nordrhein Westfalen auf das Vorhandensein von Pseudorabiesvirus- (PRV) Infektionen untersucht. Von 929 Seren, die während des 7-jährigen Beobachtungszeitraumes getestet wurden, zeigten 92 (9,4%) PRV spezifische Antikörper. Die durchschnittliche Seroprävalenz in der Eifel lag bei 11,4%. In den Jagdjahren 1996 bis 2000 wurden 473 Gewebeproben (Tonsillen, Lungen) und 516 Tupferproben (Nasal-, Genitaltupfer) von Schwarzwild aus der Eifel zwecks Virusisolierung in der Zellkultur untersucht. In der Jagdsaison 1999/00 gelang die Isolierung von 4 Viren aus den Tonsillen von Frischlingen und überläufern aus dem Landkreis Bonn (Kottenforst), die anhand desBamHI DNA-Spaltmusters als Typ Ip-Pseudorabiesviren (PRV) identifiziert werden konnten. Die Zahl der PRV-positiven Tonsillen und Lungen erhöhte sich auf 14 (4,0%) bzw. 2 (1,6%) bei der Untersuchung der Gewebeproben in einer gD-spezifischen PCR. Virusisolierung und PCR lassen persistent infizierte Frischlinge als Hauptüberträger des PRV in Schwarzwildpopulationen vermuten. Die Ergebnisse deuten weiterhin auf ein mehrjähriges endemisches Infektionsgeschehen bei Schwarzwild im Südwesten Nordrhein Westfalens hin. Nach bisherigen Erkenntnissen scheint das Untersuchungsgebiet Teil eines gro\en zusammenhängenden, endemischen Gebietes darzustellen, welches Schwarzwildpopulationen aus Frankreich, Belgien und den südwestlichen Teilen Deutschlands umfasst. Jüngst wurde die Aujeszky'sche Krankheit (AK) in den Hausschweinebeständen Nordrhein Westfalens erfolgreich getilgt und die Anerkennung als offiziell AK-freie Region erteilt. Wie in anderen Regionen Deutschlands scheinen PRV-Infektionen beim Schwarzwild die Hausschweinepopulation nicht zu gefährden.Dans le cadre d'une étude de longue haleine, un total de 1.112 sangliers d'Europe (Sus scrofa), tirés dans la partie méridionale de la Nord-Rhénanie Westphalie entre 1993 et 2000, ont été examinés quant à l'infection par le virus pseudo-rabique (PRV). Parmi 929sera testés au cours des 7 années d'obser. vation, 92 (9.4 %) présentaient des anticorps spécifiques de la PRV avec une séro-prévalence globale de 11.4 % dans le Sud-Ouest de la Nord-Rhénanie Westphalie. Un total de 473 tissus (amygdales, poumons) et de 516 prélèvements de mucosités, collectés dans ces districts entre 1996 et 2000, ont été cocultivés en vue de mettre en évidence la présence du PRV. En 2000,4 virus ont été isolés dans les amygdales de marcassins et de yearlings tirés dans le district de Bonn et ont été identifiés comme étant du type Ip PRV. Lorsque des amygdales et des poumons furent testés de faÇon complémentaire dans un PCR gD spécifique, respectivement 14 (4.0%) et 2 (1.6%) se révélèrent positifs. L'isolation des virus et les résultats au PCR suggèrent que le PRV est essentiellement transmis par les marcassins. Les résultats montrent aussi que les infections de PRV ont été endémiques dans les populations sauvages du Sanglier du Sud-Ouest de la Nord-Rhénanie-Westphalie pendant de nombreuses années. De plus, il s'avère que l'aire couverte par cette étude correspond à la bordure Nord-Ouest d'une aire considérable et cohérente sur le plan endémique, comprenant les populations de sangliers sauvages de France, Belgique et des régions adjacentes de l'Allemagne de l'Ouest. Récemment, le PRV a été éradiqué des porcs domestiques de la Nord-Rhénanie-Westphalie. Une reconnaissance comme région déclarée officiellement indemne de la maladie d'Aujesky a été sollicitée auprès de l'Union européenne mais n'a pas encore été obtenue. A l'instar d'autres régions et jusqu à présent, les infections de PRV dans la population de sangliers ne semblent pas affecter le Porc domestique. |
| Starting Page | 130 |
| Ending Page | 140 |
| Page Count | 11 |
| File Format | |
| ISSN | 00442887 |
| Journal | European Journal of Wildlife Research |
| Volume Number | 49 |
| Issue Number | 2 |
| e-ISSN | 14390574 |
| Language | French |
| Publisher | Springer-Verlag |
| Publisher Date | 2003-01-01 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Aujeszky'sche Krankheit Epidemiologie Pseudorabies Virus Seroprävalenz Sus scrofa Schwarzwild Animal Ecology Zoology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ecology Animal Science and Zoology |
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