NDLI: Conjugation of δ-endotoxin from Bacillus thuringiensis with abamectin of Streptomyces avermitilis as a new type of biocide, GCSC-BtA, for control of agricultural insect pests

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Conjugation of δ-endotoxin from Bacillus thuringiensis with abamectin of Streptomyces avermitilis as a new type of biocide, GCSC-BtA, for control of agricultural insect pests

Contributions to the knowledge of conifer aphid fauna in Turkey and their zoogeographical distribution

Journal of Pest Science : Volume 76, Issue 1, February 2003

Journal of Pest Science : Volume 75

Journal of Pest Science : Volume 74

Journal of Pest Science : Volume 73

Journal of Pest Science : Volume 72

Journal of Pest Science : Volume 71

Journal of Pest Science : Volume 70

Conjugation of δ-endotoxin from Bacillus thuringiensis with abamectin of Streptomyces avermitilis as a new type of biocide, GCSC-BtA, for control of agricultural insect pests

Content Provider	Springer Nature Link
Author	Liu, B. Sengonca, C.
Copyright Year	2003
Abstract	Conjugation of δ-endotoxin from Bacillus thuringiensis with abamectin, a toxin of Streptomyces avermitilis, was carried out to form a new type of biocide, GCSC-BtA based on “Germany-China Scientific Cooperation” research, for the control of agricultural insect pests. The strategy for biochemical linkage was designed by conjugating an amino group in B.t. protoxin with a carboxyl group in carboxylated abamectin under the treatment of conjugator EDC [1-Ethyl-3-(3-dimethylaminopropyl carbodiimide Hydrochloride)]. The formation of B.t. protoxin was processed by solubilizing B.t. crystal in 25 mM dithiothreitol (DTT) at 37 °C for 2 h. The carboxylated abamectin was formed by carboxylating the NaH-activated abamectin with 10 mg/ml butyric anhydride at 111 °C in a water-circumfluent condensation device for 2 h. The conjugating reaction, consisting of 5 mg/ml B.t. protoxin, 10 mg/ml carboxylated abamectin and 19.17 mg/ml EDC, was successfully conducted at room temperature for 24 h. Significant differences were found between pure abamectin, carboxylated abamectin and the conjugated BtA by means of UV-photo absorptions recorded at wavelengths 354, 438, 518, 600 nm (P < 0.01). LT50 of the conjugated GCSC-BtA to the 3rd instar larvae of Plutella xylostella (L.) (Lep., Plutellidae) was 35.27 μg a.i./ml, about 62 % and 76 % of that caused by the B.t. protoxin and the caxboxylated abamectin, respectively. The conjugated GCSC-BtA caused 87.14 % mortalities in larvae of P. xylostella, 93.75 % in adult Myzus persicae (Sulzer) (Hom., Aphididae) and 89.33 % in adult Phyllotreta vittata Fabricius (Col., Chrysomelidae) as compared to 48.33 % by the B.t. crystal only in P. xylostella. The symptoms caused by conjugated GCSC-BtA in the 3rd instar of P. xylostella were black color in the head part and white-yellow in the abdomen of dead larvae, which differed from the black color or the white-yellow all along the body caused by either the B.t. crystal or the abamectin, respectively. It was concluded that the conjugated GCSC-BtA biocide had a broader host spectrum and a faster killing speed than either the B.t. crystal or abamectin alone for the control of agricultural pests.
Starting Page	44
Ending Page	49
Page Count	6
File Format	PDF
ISSN	14365693
Journal	Journal of Pest Science
Volume Number	76
Issue Number	2
e-ISSN	14390280
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Insect Science

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Insecticidal activity and antifeedant effect of a new type biocide GCSC-BtA against Plutella xylostella L. (Lep., Plutellidae)

Effect of GCSC-BtA biocide on population dynamics of cabbage pests and their natural enemies from fields in southeastern China

Efficiency of the mixed biocide GCSC-BtA against vegetable pests of different arthropod orders in the south-eastern China

Effect of GCSC-BtA biocide on abundance and diversity of some cabbage pests as well as their natural enemies in southeastern China

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Unusual Proteolytic Processing of a δ-Endotoxin from Bacillus thuringiensis Strain Buibui by Larval Midgut-Juice of Anomala cuprea HOPE (Coleoptera: Scarabaeidae)

Fractionation and Some Features of Synergistic Compound Which Enhances the Insecticidal Activity of δ-Endotoxin of Bacillus thuringiensis

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Toxicity of biocide GCSC-BtA on arthropod pests under different temperature conditions

Conjugation of δ-endotoxin from Bacillus thuringiensis with abamectin of Streptomyces avermitilis as a new type of biocide, GCSC-BtA, for control of agricultural insect pests

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