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| Content Provider | Springer Nature Link |
|---|---|
| Author | Schlyter, Fredrik Marling, Elisabeth Löfqvist, Jan |
| Copyright Year | 2004 |
| Abstract | A micro-feeding assay was developed for testing small amounts of synthetics and extracts as antifeedants for Hylobius pine weevils. We devised a simple setup consisting of 5×5-mm pieces of thin layer chromatography cellulose plates used in pairs for a bioassay involving choice. After application of 1.5 μl solutions and evaporation of the solvent, each plate received a 5 μl aliquot of 1 M sucrose as a feeding stimulant. The test period was 4 h at 25 °C with 6 days of starvation before the test, compared to just the 1 day sufficient for twig tests. Deprivation of water for 1 day at 65% RH before the test was critical because it increased feeding but not mortality. The combination of starvation and thirst reduced the relative variance (coefficient of variation) to below 50%. There was a good correlation of antifeedant activity in the microassay with the activity in a standard twig test. The microassay needs only several mg for a test series, whereas the twig test needs grams. |
| Starting Page | 191 |
| Ending Page | 195 |
| Page Count | 5 |
| File Format | |
| ISSN | 16124758 |
| Journal | Journal of Pest Science |
| Volume Number | 77 |
| Issue Number | 4 |
| e-ISSN | 16124766 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2004-03-23 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Pine weevils Antifeedants Microassay |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ecology Plant Science Ecology, Evolution, Behavior and Systematics Insect Science Agronomy and Crop Science |
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