NDLI: Warfarin resistance test and polymorphism screening in the VKORC1 gene in Rattus flavipectus

Content Provider	Springer Nature Link
Author	Huang, Bao Huan Feng, Zhi Yong Yue, Ling Fen Yao, Dan Dan Gao, Zhi Xiang Wang, Da Wei Liu, Xiao Hui
Copyright Year	2010
Abstract	The buff-breasted rat (Rattus flavipectus) is a major agricultural pest across China. Warfarin-resistant animals have been found in several major provinces in China, and are hampering effective control. Molecular mechanisms underlying resistance to anticoagulant rodenticides have been determined for other species, but genetic information regarding resistance in R. flavipectus remains unknown. The vitamin K 2,3-epoxide reductase complex subunit 1 (VKORC1) encoded by VKORC1 gene is the molecular target of coumarin anticoagulants, and amino acid substitutions in VKORC1 coding-regions have been reported as one of the supposed mechanisms of warfarin resistance. Here, lethal feeding test in R. flavipectus (n = 36) was conducted in Zhanjiang, China. Four animals (11%) survived the test period of 25 days and were identified as warfarin resistance. Polymorphism across the whole genome DNA sequence of the VKORC1 gene was screened out and compared with resistant and non-resistant rats. A total of nine single nucleotide polymorphisms (SNPs) were identified including seven SNPs in introns and two SNPs in exons, and the SNP (2317A > G) located in exon 3 led to the amino acid substitution (Tyr139Cys) in VKORC1 protein. Based on the characteristics of Tyr139Cys mutation of VKORC1 in humans or rats and its relationship with warfarin-resistance, Tyr139Cys mutation may be one mutation responsible for anticoagulant resistance in R. flavipectus. Given the low numbers of resistant rats in our feeding test, wider surveillance, tests of resistance development in a larger wild population and further researches on the genetic mechanisms of anticoagulant resistance in R. flavipectus are necessary.
Starting Page	87
Ending Page	92
Page Count	6
File Format	PDF
ISSN	16124758
Journal	Journal of Pest Science
Volume Number	84
Issue Number	1
e-ISSN	16124766
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-09-24
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Rattus flavipectus Warfarin-resistance test VKORC1 SNP Forestry Entomology Plant Sciences Ecology Agriculture Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Ecology Plant Science Ecology, Evolution, Behavior and Systematics Insect Science Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Behavioral response of Norway rats (Rattus norvegicus) to odors of different mammalian species

Field evidence of roof rat (Rattus rattus) faecal contamination of barley grain stored in silos in the Czech Republic

The Betula aphids of Turkey

Seasonal variation in population characteristics and management implications for brown rats (Rattus norvegicus) within their native range in Harbin, China

Better food-based baits and lures for invasive rats Rattus spp. and the brushtail possum Trichosurus vulpecula: a bioassay on wild, free-ranging animals

Behavioral responses of Thrips tabaci Lindeman to endophyte-inoculated onion plants

Erratum to: When host-plant resistance to a pest leads to higher plant damage

News from the Editor-in-Chief

Resistance of tropical maize genotypes to the larger grain borer

Warfarin resistance test and polymorphism screening in the VKORC1 gene in Rattus flavipectus

Similar Documents

Behavioral response of Norway rats (Rattus norvegicus) to odors of different mammalian species

Field evidence of roof rat (Rattus rattus) faecal contamination of barley grain stored in silos in the Czech Republic

The Betula aphids of Turkey

Seasonal variation in population characteristics and management implications for brown rats (Rattus norvegicus) within their native range in Harbin, China

Better food-based baits and lures for invasive rats Rattus spp. and the brushtail possum Trichosurus vulpecula: a bioassay on wild, free-ranging animals

Behavioral responses of Thrips tabaci Lindeman to endophyte-inoculated onion plants

Erratum to: When host-plant resistance to a pest leads to higher plant damage

News from the Editor-in-Chief

Resistance of tropical maize genotypes to the larger grain borer

Warfarin resistance test and polymorphism screening in the VKORC1 gene in Rattus flavipectus