NDLI: Identification and Quantification of Aconitines and Colchicine in Serum, Urine, and Plants using MonoSpin C18 and LC-MSMS

Content Provider	Springer Nature Link
Author	Saito, Takeshi Umebachi, Rimako ra, Akira Oikawa, Hiroshi Miyazaki, Shota Fujita, Yuji Ikuchi, Sadaki
Copyright Year	2015
Abstract	Aconitum and Colchicum are known to be highly toxic plants based on their respective toxins, namely aconite and colchicine. Aconite and colchicine poisoning generally occurs because of misidentification and contamination, and is also associated with homicide and suicide cases. The detection of aconitines and colchicine in body fluids and leftovers plays an important role in clinical and forensic toxicology investigation of suspected food poisoning by natural poisons. Therefore, we have developed and validated a rapid determination of aconitine, hypaconitine, jesaconitine, mesaconitine, and colchicine in serum, urine, and plants using liquid chromatography–tandem mass spectrometry. All targeted compounds, together with internal standards, were extracted from serum and urine using a C18 monolithic spin column. Recovery of all compounds from serum, spiked at concentrations of 0.1, 0.8, 6, and 18 ng mL−1, ranged from 96.8 to 101.4 %. Recovery of all compounds from urine, spiked at concentrations of 0.1, 8, 80 and 190 ng mL−1, ranged from 96.6 to 101.9 %. The intra- and inter-assay accuracy and precision (expressed as relative standard deviation, %RSD) evaluated for all compounds were within 96.7–103.2 % and 1.7–11.5 %, respectively. Detection limits and quantitation limits of aconitines and colchicine for serum and urine were 0.05 and 0.1 ng mL−1, respectively. This method had a chromatographic run time of 7.0 min. Calibration curves for serum and urine ranged from 0.1 to 20 ng mL−1 for serum and 0.1–200 ng mL−1 for urine, respectively. The validated method was successfully applied to clinical aconitine and colchicine poisoning cases.
Starting Page	1041
Ending Page	1048
Page Count	8
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	78
Issue Number	15-16
e-ISSN	16121112
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-06-17
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Aconitines Colchicine Monolithic spin column LC—MS/MS Chromatography Proteomics Pharmacy Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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