NDLI: Adsorptive BSA Coating Method for CE to Separate Basic Proteins

Content Provider	Springer Nature Link
Author	Man, Yan Lv, XueFei Iqbal, Javed Jia, Fuchao Xiao, Peng Hasan, Murtaza Li, Qin Dai, Rongji Geng, Lina Qing, Hong Deng, Yulin
Copyright Year	2012
Abstract	A novel and simple coating method was developed by coating bovine serum albumin (BSA) onto the inner surface of a fused-silica capillary, to avoid the adsorption of analytes during CE. The advantage presented here was that the coating process is more simple, fast, stable, and reproducible. The coated capillary avoided the adsorption of analytes onto the inner surface of a fused-silica capillary and might be a promising candidate for separation of complex biological samples with further development. Meanwhile, the efficiencies of the coated capillary were evaluated by EOF, chromatographic peak shape, and theoretical plate number (N m−1) of RNase A. The optimal coating conditions were obtained from the results. The pH value of coating buffer PB was 4.2, the standing time was 12 h at 4 °C, and the coating concentration of BSA was 1.5 mg mL−1. The stability of the coating on the inner wall of the capillary and the reproducibility of the coated capillaries were good. The theoretical plate number values of RNase A were over 1.3 × 105 (N m−1) in the coated capillary. After successive electrophoresis for 48 h using the coated capillary, the RSD values of EOF and the theoretical plate number were 4.14 % and 9.14 %, respectively. In addition, the RSD values of EOF and the theoretical plate number (N m−1) in the coated capillaries were 13.19 % and 8.96 %, respectively. Finally, the coated capillary was successfully applied to separate the mixture of four basic proteins (RNase A, lysozyme, trypsin and myoglobin).
Starting Page	59
Ending Page	65
Page Count	7
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	76
Issue Number	1-2
e-ISSN	16121112
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-12-05
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Capillary electrophoresis BSA coating Adsorption Basic proteins separation Chromatography Proteomics Pharmacy Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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