NDLI: Sample Treatment Approaches for Trace Level Determination of Cesium in Hepatitis B Vaccine by Suppressed Ion Chromatography

Content Provider	Springer Nature Link
Author	Dash, Kulamani Thangavel, Shanmugam Rastogi, Lori Dhavile, Shrinivas M. Arunachalam, Jayaraman
Copyright Year	2011
Abstract	The hepatitis B surface antigen manufactured by recombinant DNA technology is extracted from the culture media by density gradient centrifugation using cesium salts. Cesium is considered to be toxic, because it affects active ion transport by blocking potassium channels. The residual trace levels of cesium in hepatitis B vaccine samples are determined by suppressed ion chromatography. Hepatitis B vaccines contain various buffer salts, aluminum-containing adjuvants, proteins and traces of iron. The polyvalent cations (Al3+, Fe3+) and proteins degrade the chromatographic performance in terms of decreased retention time and poor reproducibility. Different sample preparation approaches were evaluated with the aim of eliminating these foulants: (1) filtration, (2) digestion and (3) digestion-protein precipitation. Quantitative elimination of these foulants was achieved in the digestion-protein precipitation sample clean-up approach. Cesium was separated on the IonPac CS17 column with suppressed conductivity detection. The results of the ion chromatography (IC) method were compared with ICP-MS analysis. The precision of determination was better than 6.5% (relative standard deviation) with a method detection limit of 45 ng mL−1. The expanded uncertainty in the measurement at 95% confidence level (coverage factor 2) is better than 16.3%.
Starting Page	17
Ending Page	23
Page Count	7
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	75
Issue Number	1-2
e-ISSN	16121112
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-11-08
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Hepatitis B vaccine Cesium Polyvalent cations Proteins Suppressed ion chromatography Proteomics Analytical Chemistry Pharmacy Organic Chemistry Biochemistry Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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