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| Content Provider | Springer Nature Link |
|---|---|
| Author | Destandau, Emilie Lesellier, Eric |
| Copyright Year | 2008 |
| Abstract | For almost 10 years, silica-based monolithic stationary phases have been used in numerous fields of analytical chemistry, especially when high flow rate is required or for the separation of high molecular weight compounds such as peptides. In comparison with particulate stationary phases, the macroporous structure of the silica rod induces low pressure drops, which allows the use of high flow rates, leading to a dramatic reduction of the analysis duration. We suggest another use of this type of phases with mobile phases having a high viscosity, such as ethanol–water mixtures. These mobile phases are environmentally friendly, avoiding the use of toxic solvents such as methanol and acetonitrile, and consequently the necessary cleaning of waste is reduced. In this paper, the eluting strength of different hydro-organic mobile phases and their efficiencies are compared on Chromolith RP-18e. Due to higher eluting strength, the use of ethanol–water mixtures also allows decreasing the amount of organic solvent required to achieve a separation, whereas, despite a higher viscosity, the chromatographic efficiencies are as good as those obtained with classical mobile phases. Finally, separation of pesticides (triazines) and sunscreen molecules are presented. |
| Starting Page | 985 |
| Ending Page | 990 |
| Page Count | 6 |
| File Format | |
| ISSN | 00095893 |
| Journal | Chromatographia |
| Volume Number | 68 |
| Issue Number | 11-12 |
| e-ISSN | 16121112 |
| Language | English |
| Publisher | Vieweg Verlag |
| Publisher Date | 2008-10-01 |
| Publisher Place | Wiesbaden |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Column liquid chromatography Green chromatography Ethanol–water mobile phases Silica-based monolithic columns Triazine pesticides Sunscreens Measurement Science, Instrumentation Plant Sciences Biochemistry Pharmacy Organic Chemistry Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry |
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