NDLI: Measurement Bias on Nanoparticle Size Characterization by Asymmetric Flow Field-Flow Fractionation Using Dynamic Light-Scattering Detection

Content Provider	Springer Nature Link
Author	Gigault, Julien Mignard, Emmanuel Hadri, Hind El Grassl, Bru
Copyright Year	2017
Abstract	In this work, we highlight the influence of the particle–particle interaction on the retention behavior in asymmetric flow field-flow fractionation (A4F) and the misunderstanding considering the size determination by a light-scattering detector (static and dynamic light scattering) by comparing fullerene nanoparticles to similar sized polystyrene nanoparticle standards. The phenomena described here suggest that there are biases in the hydrodynamic size and diffusion determination induced by particle–particle interactions, as characterized by their virial coefficient. The dual objectives of this paper are to (1) demonstrate the uncertainties resulting from the current practice of size determination by detectors coupled to an A4F system and (2) initiate a discussion of the effects of particle–particle interactions using fullerene nanoparticles on their characterization as well as their origins. The results presented here clearly illustrate that the simple diffusion coefficient equation that is generally used to calculate the hydrodynamic size of nanoparticles (NPs) cannot be considered for whole fractograms according to their size distribution. We tried to identify particle interactions that appear during fractionation and demonstrated using the fully developed diffusion coefficient equation. We postulate that the observed interaction-dependent retention behavior may be attributed to differences in the virial coefficient between NPs and between NPs and the accumulation wall (membrane surface) without quantifying it. We hope that our results will stimulate discussion and a reassessment of the size determination procedure by A4F-LS to more fully account for all the influential material parameters that are relevant to the fractionation of nanoscale particles by A4F.
Starting Page	287
Ending Page	294
Page Count	8
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	80
Issue Number	2
e-ISSN	16121112
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2017-01-23
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Asymmetric flow field-flow fractionation Light scattering Nanoparticles Fullerenes Size characterization Chromatography Proteomics Pharmacy Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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