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| Content Provider | Springer Nature Link |
|---|---|
| Author | Aguete, E. C. Gago Martínez, A. Rodríguez Vázquez, J. A. O'Connell, S. Moroney, C. James, K. J. |
| Copyright Year | 2001 |
| Abstract | High-performance liquid chromatography (HPLC) and different high-performance capillary electrophoresis (HPCE) methods have been developed for the detection and quantitation of cyanobacterial toxins in environmental samples.Cyanobacteria, also known as blue-green algae, are responsible for toxic contamination of fresh water. Several serious episodes involving microcystins have been reported in recent years; the most common and toxic of these hepatotoxins is microcystin-LR (MC-LR). Sensitive analytical methodologies are required for determination of these compounds because of the implications for human health when the toxins are present in drinking water.Different modes of operation of HPCE were used and the results obtained were compared with those obtained by HPLC. Citrate buffer resulted in good separation of the microcystins when capillary electrophoresis was used in capillary zone electrophoresis (CZE) and capillary isotachophoresis (CITP) modes; borate buffer containing sodium dodecyl sulphonate (SDS) was used in micellar electrokinetic chromatography (MEKC). Conditions were optimized and the methods were applied to water samples and cyanobacterial cells containing microcystins. Results obtained by use of HPLC-UV and different modes of operation of CE-UV were compared in terms of sensitivity, selectivity, and efficiency. |
| Starting Page | S254 |
| Ending Page | S259 |
| File Format | |
| ISSN | 00095893 |
| Journal | Chromatographia |
| Volume Number | 53 |
| Issue Number | 1 |
| e-ISSN | 16121112 |
| Language | English |
| Publisher | Vieweg Verlag |
| Publisher Date | 2001-01-01 |
| Publisher Place | Wiesbaden |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Column liquid chromatography Micellar electrokinetic chromatography Capillary electrophoresis Isotachophoresis Microcystins Analytical Chemistry Organic Chemistry Pharmacy Biochemistry Plant Sciences Measurement Science, Instrumentation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry |
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