NDLI: Fast and Precise Quantification of l-Homoarginine in Human Plasma by HILIC-Isotope Dilution-MS

Content Provider	Springer Nature Link
Author	Martens Lobenhoffer, Jens Surdacki, Andrzej Bode Böger, Stefanie M.
Copyright Year	2013
Abstract	Lowered plasma concentrations of the endogenous amino acid l-homoarginine have been recently identified as an independent risk factor for cardiovascular and all-cause mortality in patients referred for coronary angiography in the LURIC study. To support further investigations into this matter, we describe here a fast and easy LC–MS–MS method for the detection of l-homoarginine in human plasma. The sample preparation consisted only of the addition of the stable isotope-labeled internal standard d 4-l-homoarginine and protein precipitation. The analytes were separated isocratically on an HILIC silica column. Detection took place by tandem mass spectrometry. The calibration function was linear in the range of 0.1–10 μmol L−1. The intra-day precision was better than 2 % RSD and the inter-day precision better than 4 % RSD in plasma. The accuracy was always better than 5 % deviation. The method was matrix independent owing to the usage of the analogous stable isotope-labeled internal standard.
Starting Page	1755
Ending Page	1759
Page Count	5
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	76
Issue Number	23-24
e-ISSN	16121112
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-09-21
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Hydrophilic interaction liquid chromatography–tandem mass spectrometry Stable isotope dilution l-Homoarginine Human plasma Chromatography Proteomics Pharmacy Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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