NDLI: A Rapid and Sensitive LC–MS–MS Method for Determination of Hepcidin-25 in Human Serum, and Measurement of its Diurnal Rhythm for Healthy Subjects

Content Provider	Springer Nature Link
Author	Zheng, Xin Chen, Xia Jian, Nancheng Chen, Jia Hu, Pei Jiang, Ji
Copyright Year	2014
Abstract	Hepcidin is a recently discovered small defensin-like peptide hormone which is a key regulator of systemic iron homeostasis. Serum and urine hepcidin levels may be of diagnostic importance for iron-related disorders. We have developed a sensitive and selective ultra-performance liquid chromatographic–tandem mass spectrometric (UPLC–MS–MS) method for quantitative determination of the concentration of hepcidin in clinical samples. Solid-phase extraction was used to isolate the analyte from biological matrices, and the extracts were injected on to a Waters Acquity UPLC BEH C18 column with gradient elution. Detection was performed by triple-quadrupole tandem mass spectrometry in multiple reaction monitoring mode with positive electrospray ionization. This UPLC–MS–MS method was validated by use of a recently introduced validation strategy based on accuracy profiles. Accuracy, precision, and linearity were good in the concentration range 0.50–500 ng mL−1. The lower limit of quantification was 0.50 ng mL−1 serum. A diurnal rhythm of serum hepcidin-25 levels was found by standardizing the sampling time from 07:30 on one day to 07:30 on the third day for eight healthy volunteers. The results indicated that circulating hepcidin-25 concentrations are different between genders and for different diurnal rhythms.
Starting Page	73
Ending Page	80
Page Count	8
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	78
Issue Number	1-2
e-ISSN	16121112
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-12-11
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	UPLC–MS–MS Hepcidin-25 MRM Diurnal rhythm Chromatography Proteomics Pharmacy Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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