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| Content Provider | Springer Nature Link |
|---|---|
| Author | Liu, H. L. Kao, T. H. Chen, B. H. |
| Copyright Year | 2004 |
| Abstract | The amount and variety of carotenoids in Jiao-Gu-Lan (Gynostemma pentaphyllum MAKINO), a traditional Chinese medicinal herb which is currently sold in Taiwan and China as herbal tea, were determined by HPLC. Dried Jiao-Gu-Lan samples were ground into fine materials, and the carotenoids were extracted, saponified and subjected to HPLC analysis. A gradient mobile phase of methanol/isopropanol (99/1, v/v) (A) and methylene chloride (B) was used: 100% A and 0% B initially, maintained for 15 min, decreased to 70% A in 45 min, maintained for 15 min, and returned to 100% A in 65 min. A total of 25 carotenoids were resolved within 54 min by using a C-30 column with flow rate at 1.0 mL min−1 and detection at 450 nm. For all the six cultivars of Jiao-Gu-Lan samples, all-trans-lutein was present in largest amount (72–376 μg g−1), followed by cis isomers of lutein (0–72 μg g−1), neochrome (13–65 μg g−1), neoxanthin (0–41 μg g−1), all-trans- α-carotene (0–27 μg g−1), auroxanthin (7–23 μg g−1), violaxanthin (3–22 μg g−1), luteoxanthin (4–19 μg g−1), all-trans-β-carotene (0–7.6 μg g−1), β-cryptoxanthin (0–6.7 μg g−1), and cis isomers of β-carotene (0–1.9 μg g−1), on a dry weight basis. |
| Starting Page | 411 |
| Ending Page | 417 |
| Page Count | 7 |
| File Format | |
| ISSN | 00095893 |
| Journal | Chromatographia |
| Volume Number | 60 |
| Issue Number | 7-8 |
| e-ISSN | 16121112 |
| Language | English |
| Publisher | Vieweg Verlag |
| Publisher Date | 2004-09-16 |
| Publisher Place | Wiesbaden |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Column liquid chromatography Carotenoids Jiao-Gu-Lan plant Gynostemma Pentaphyllum |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry |
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