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| Content Provider | Springer Nature Link |
|---|---|
| Author | Tripodi, V. P. Lucangioli, S. E. Barbara, C. L. Rodríguez, V. G. Carducci, C. N. |
| Copyright Year | 2001 |
| Abstract | A simple and practical micellar electrokinetic capillary chromatography (MEKC) method is proposed for the quantitation of immunosuppressive drugs such as azathioprine (AZA), mycophenolate mofetil (MMF), cyclosporine A (CyA) and tacrolimus (FK 506). The electrophoretic separation of the analytes was performed with a background electrolyte containing 20 mM phosphate buffer at pH 7.5, 50 mM SDS and methanol as an organic modifier. Fused silica capillaries 75 μm i.d. and 60 cm in length were employed and detection of analytes was performed at 214 nm.Thorough validation according to international guidelines showed that the proposed method is reliable and appropiate for the routine analysis of immunosuppressants. Moreover, it may be an advantageous alternative to the traditional chromatographic methodologies currently employed in the pharmaceutical and bioanalysis fields. |
| Starting Page | 93 |
| Ending Page | 98 |
| Page Count | 6 |
| File Format | |
| ISSN | 00095893 |
| Journal | Chromatographia |
| Volume Number | 54 |
| Issue Number | 1-2 |
| e-ISSN | 16121112 |
| Language | English |
| Publisher | Vieweg Verlag |
| Publisher Date | 2001-01-01 |
| Publisher Place | Wiesbaden |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Micellar electrokinetic capillary chromatography Immunosuppressive drugs Cyclosporine A Azathioprine Mycophenolate mofetil Tacrolimus Analytical Chemistry Organic Chemistry Pharmacy Biochemistry Plant Sciences Measurement Science, Instrumentation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry |
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