NDLI: Metabolite Profiling of In Vitro Cultured and Field Grown Rhizomes of Acorus calamus from Mongolia Using GC

Content Provider	Springer Nature Link
Author	Deepalakshmi, Putchen Dakshinamoorthy Odgerel, Khongorzul Thirugnanasambantham, Pankajavalli Yungeree, Oyunbileg Khorolragchaa, Altanzul Senthil, Kalaiselvi
Copyright Year	2016
Abstract	Acorus calamus (sweet flag) is used in the traditional Chinese and Indian medicines for various ailments. Due to its extensive use in herbal medicine, natural resources from the world’s forests are being depleted at an alarming rate. In the present study, an in vitro cell culture technique is being explored as an alternative to field grown A. calamus with respect to the metabolite profile, antioxidant properties, total phenol, and total flavonoid content. Gas chromatography mass spectrometry (GC–MS) was utilized to compare the metabolite profiling between methanolic extracts of in vitro and field grown rhizome tissues of A. calamus. A statistical analysis indicated an upregulation of α-selinene, which is representative of sesquiterpene ketones, and a cyclic polyol, d-pinitol, which has an insulin mimicking effect in the in vitro cultivated rhizome tissue when compared to field grown rhizomes. Significantly higher free-radical scavenging activity (IC50 69.32 μg mL−1), total phenolic content (71.60 mg GAE g−1), and total flavonoid content (42.34 mg CE g−1) were observed in in vitro rhizome tissues compared with those from field grown rhizomes. These observations suggest that the in vitro cultivation of Acorus rhizomes could be exploited as an alternative to field grown A. calamus, as it is an endangered medicinal plant. The production of useful metabolites by the in vitro cultured rhizomes can be explored successfully for utilization by various food and drug industries.
Starting Page	1359
Ending Page	1371
Page Count	13
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	79
Issue Number	19-20
e-ISSN	16121112
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-08-24
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Metabolic profiling Acorus calamus Rhizome GC–MS Free-radical scavenging Chromatography Proteomics Pharmacy Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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