NDLI: Hybridization Assay by Time-Resolved Capillary Gel Electrophoresis with a Lanthanide Chelate

Content Provider	Springer Nature Link
Author	Sumitomo, Keiko Ito, Takahisa Sasaki, Motoyasu Yamaguchi, Yoshiri
Copyright Year	2008
Abstract	Capillary electrophoresis of crude biological samples with time-resolved fluorescence (TRF) detection enables elimination of interference from organic fluorophores and from light scattering. Because the fluorescence lifetime of biological substances and impurities overlaps the fluorescence lifetime of conventional labeling dyes, TRF detection with conventional organic labeling dyes suffers from background fluorescence. In this work, we synthesized a luminescent lanthanide chelating reagent to covalently bind the 5′-end of DNA through its dichroic functional group while retaining the unique luminescent properties of the lanthanide chelate, i.e. large Stokes shift, sharp emission, and a long luminescence lifetime in the microsecond to millisecond range. The luminescence of lanthanide chelates is inherently quenched by dissociation of the central metals in typical biological buffers containing a strong chelator, for example EDTA or phosphate; the synthesized Eu3+ chelate reagent, however, was stable even in EDTA solutions. In addition to stability in biological buffer solution, the synthesized Eu3+ chelate reagent enabled direct labeling of single-stranded oligonucleotides, and was used for DNA hybridization assay by time-resolved capillary gel electrophoresis. DNA hybridization assay in fetal bovine serum was also demonstrated.
Starting Page	715
Ending Page	721
Page Count	7
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	67
Issue Number	9-10
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	2008-04-18
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Capillary electrophoresis Time-resolved fluorescence (TRF) Lanthanide chelate Hybridization assay Measurement Science, Instrumentation Plant Sciences Biochemistry Pharmacy Organic Chemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Analysis of glutamate in beverages and foodstuffs by capillary electrophoresis with laser-induced fluorescence detection

Quantitative assay for neohesperidin dihydrochalcone in foodstuffs by capillary electrophoresis

Ultrasensitive Capillary Electrophoresis of Oligoguluronates with Laser-Induced Fluorescence Detection

Determination of baclofen by derivatization with anthracene-2,3-dicarboxaldehyde followed by capillary electrophoresis with laser-induced fluorescence detection

Capillary gel electrophoresis of oligonucleotides using polymer solutions

Determination of Genetically Modified Soybean by Multiplex PCR and CGE with LIF Detection

Enantioseparation of venlafaxine andO-desmethylvenlafaxine by capillary electrophoresis with mixed cyclodextrins

High Resolution Capillary Electrophoresis of Oligosaccharide Structural Isomers

Thermostating in capillary electrophoresis

Hybridization Assay by Time-Resolved Capillary Gel Electrophoresis with a Lanthanide Chelate

Similar Documents

Analysis of glutamate in beverages and foodstuffs by capillary electrophoresis with laser-induced fluorescence detection

Quantitative assay for neohesperidin dihydrochalcone in foodstuffs by capillary electrophoresis

Ultrasensitive Capillary Electrophoresis of Oligoguluronates with Laser-Induced Fluorescence Detection

Determination of baclofen by derivatization with anthracene-2,3-dicarboxaldehyde followed by capillary electrophoresis with laser-induced fluorescence detection

Capillary gel electrophoresis of oligonucleotides using polymer solutions

Determination of Genetically Modified Soybean by Multiplex PCR and CGE with LIF Detection

Enantioseparation of venlafaxine andO-desmethylvenlafaxine by capillary electrophoresis with mixed cyclodextrins

High Resolution Capillary Electrophoresis of Oligosaccharide Structural Isomers

Thermostating in capillary electrophoresis

Hybridization Assay by Time-Resolved Capillary Gel Electrophoresis with a Lanthanide Chelate