NDLI: Determination of Phenolic Acids in Herba Epilobi by ITP–CE in the Column-Coupling Configuration

Content Provider	Springer Nature Link
Author	Šafra, J. Pospíšilová, M. Spilková, J.
Copyright Year	2006
Abstract	The combination of capillary isotachophoresis (ITP) and capillary zone electrophoresis (CZE) in the column-coupling configuration has been optimized in a mode in which the background electrolyte employed in the CZE step was different from the leading and terminating electrolytes of the ITP step. The optimum composition of the electrolyte system was 0.01 M HCl, 0.02 M IMI, 0.2% HEC, pH 7.2 (leading electrolyte), 0.01 M HEPES, pH 8.2 (terminating electrolyte), and 25 mM MES, 50 mM TRIS, 30 mM boric acid, 0.2% HEC, pH 8.3 (background electrolyte). All solutions contained 20% methanol. The timing of the transfer of isotachophoretically stacked analyte zones into the CZE column was also optimized. An ITP–CZE method with UV detection at 270 nm was developed for separation of nine phenolic acids (protocatechuic, syringic, vanillic, cinnamic, ferulic, caffeic, ρ-coumaric, chlorogenic, and gentisic acids) in a model mixture and used for assay of some of these acids in a methanolic extract of herba epilobi. Application of ITP–CZE resulted in 100-fold better sensitivity than conventional CZE; limits of detection ranged between 10 and 60 ng mL−1. When MES–TRIS–borate-based buffer, pH 8.3, was used in the CZE separation step the linearity of the ITP–CZE response was satisfactory (correlation coefficients were from 0.9937 to 0.9777). Repeatability was also satisfactory (RSD values ranged between 0.77% and 1.28% for migration times and between 1.65% and 13.69% for peak area).
Starting Page	37
Ending Page	43
Page Count	7
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	64
Issue Number	1-2
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	2006-07-05
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Capillary isotachophoresis Zone electrophoresis Phenolic acids Plant extract Epilobium Analytical Chemistry Organic Chemistry Biochemistry Plant Sciences Pharmacy Measurement Science, Instrumentation
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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Determination of Phenolic Acids in Herba Epilobi by ITP–CE in the Column-Coupling Configuration