NDLI: Direct coupling of capillary electrophoresis and nuclear magnetic resonance spectroscopy for the identification of a dinucleotide

Content Provider	Springer Nature Link
Author	Schewitz, J. Pusecker, K. Gfrörer, P. Götz, U. Tseng, L. H. Albert, K. Bayer, E.
Copyright Year	1999
Abstract	Coupling of capillary electrophoresis (CE) with nuclear magnetic resonance (NMR) spectroscopy has been achieved by use of a recently developed capillary NMR interface. The technique has been used for the analysis of synthetic nucleic acids. A crude adenosine dinucleotide synthesized by solid-phase chemistry has been investigated by continuous-flow CE-NMR. The combination of the separation technique and NMR spectroscopy proved well suited to identification of the main components, and to detection of by-products of the synthesis of the nucleotide. Two-dimensional CE-NMR spectra were also acquired in the stopped-flow mode and the TOCSY NMR spectrum thus acquired afforded confirmatory evidence for the structure of the adenosine dinucleotide. All experiments were performed in fully deuterated solvents and using nanoliter amounts of the dinucleotide sample.
Starting Page	333
Ending Page	337
Page Count	5
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	50
Issue Number	5-6
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	1999-01-01
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Capillary electrophoresis Nuclear magnetic resonance spectroscopy Hyphenation Nucleic acids Analytical Chemistry Organic Chemistry Pharmacy Biochemistry Plant Sciences Measurement Science, Instrumentation
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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