NDLI: Monolithic Spin Column Extraction and GC-MS for Simultaneously Detecting Nine Cold Medication Compounds and the Drug Bromoisovaleryl Urea in Human Serum

Content Provider	Springer Nature Link
Author	Saito, Takeshi Yamagiwa, Takeshi Kishiyama, Izumi Miyazaki, Shota Nakamoto, Akihiro Nishida, Manami ra, Akira Ikuchi, Sadaki
Copyright Year	2009
Abstract	We describe and validate a gas chromatography-mass spectrometry (GC-MS) method for the simultaneous quantitative detection of nine cold medication compounds and bromoisovaleryl urea an over-the-counter cold medication, in human serum; the nine compounds are acetaminophen (APAP), codeine, dihydrocodeine, three ephedrines, ethenzamide, ibuprofen, and salicylic acid. After adding the internal standard, acetaminophen-d 4 (APAP-d 4), compounds were extracted from the serum samples in the monolithic spin column. The extracted samples were evaporated to dryness. The residues were derivatized with acetonitrile and N-methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide + 1% tert-butyldimethylchlorosilane, which were subjected to GC-MS analysis. The limit of quantification (LOQ) was 0.005–0.1 μg mL−1. The calibration curves were linear (r 2 > 0.995) in the concentration range from the LOQ to 10 μg mL−1. The intra- and inter-day variations, determined by the measurement of quality control samples at three tested concentrations, showed acceptable values. The lower limit of detection was between 0.005 and 0.05 μg mL−1. Mean recoveries from the serum samples were between 2.5 and 73.8%. This procedure was applied in the toxicological analysis of an intoxicated patient who was responsible for a traffic accident.
Starting Page	519
Ending Page	526
Page Count	8
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	70
Issue Number	3-4
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	2009-07-08
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Gas chromatography-mass spectrometry Monolithic spin column extraction Cold medicine Bromoisovaleryl urea Measurement Science, Instrumentation Plant Sciences Biochemistry Pharmacy Organic Chemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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