NDLI: SDS-PAGE as a Tool for Hydrodynamic Diameter-Dependent Separation of Quantum Dots

Content Provider	Springer Nature Link
Author	Krizkova, Sona Dostalova, Simona Michalek, Petr Nejdl, Lukas Kominkova, Marketa Milosavljevic, Vedran Moulick, Amitava Vaculovicova, Marketa Kopel, Pavel Adam, Vojtech Kizek, Rene
Copyright Year	2015
Abstract	The purpose of this study was to test quantum dots (QDs) separation by native and Tris–Glycine SDS-PAGE according to the protocols commonly used for protein analyses. To study the electrophoretic behaviour of quantum dots, ten samples of previously synthesized CdTe QDs stabilized with mercaptosuccinic acid (MSA) were used. Prior to electrophoresis the hydrodynamic diameters of QDs and zeta potentials were determined, as well as the fluorescence properties and stability of QDs in the running buffers. After verification of QDs stability and separation in native polyacrylamide gel, SDS-PAGE in gradient 4–20 % polyacrylamide gel was performed. Under UV irradiation a colour-dependent separation of QDs was observed, which was consistent with their hydrodynamic diameter distribution. The electrophoretic conditions were further optimized with respect to achieving the optimal colour separation, fluorescence stability and to minimize the time of analysis. Based on the results obtained, for further work 15 % polyacrylamide gels with SDS were used and the times (30–60 min) and voltage (100–150 V) used for separation were optimized. Under the optimal separation conditions (30 min, 100 V) the addition of MSA in the concentration range 0–4 mM was used to improve visualization of QDs with diameters in the range from 7 ± 2 to 4 ± 2 nm.
Starting Page	785
Ending Page	793
Page Count	9
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	78
Issue Number	11-12
e-ISSN	16121112
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-04-18
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Quantum dots SDS-PAGE Electrophoresis Separation Hydrodynamic diameter-dependent Chromatography Proteomics Pharmacy Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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