NDLI: Hyphenated HSCCC–DPPH· for Rapid Preparative Isolation and Screening of Antioxidants from Selaginella moellendorffii

Content Provider	Springer Nature Link
Author	Shi, Shuyun Zhou, Honghao Zhang, Yuping Huang, Kelong
Copyright Year	2008
Abstract	A method for rapid preparative isolation and screening of antioxidants has been developed by combining preparative HSCCC with on-line radical scavenging detection by use of DPPH· (1,1-diphenyl-2-picrylhydrazyl) as model radical. The HSCCC effluent was split into two parts. The major part was collected and the rest was introduced, through a switch valve, directly into a reaction coil for on-line evaluation of antioxidant activity. Each of the antioxidants separated by HSCCC reacted postcolumn with the DPPH· solution in methanol and was observed as a negative peak at 517 nm of size corresponding to its antioxidant activity. The on-line method was successfully used for preparative isolation and screening of antioxidant components from an ethyl acetate extract of the traditional Chinese medicine Selaginella moellendorffii. A total of 114.7 mg amentoflavone, 12.4 mg hinokiflavone, 7.2 mg podocarpusflavone A, and 1.1 mg ginkgetin, identified as the main radical scavengers, were obtained with purity of 99.4, 98.6, 98.9, and 99.2%, respectively, as determined by LC, from 1.0 g ethyl acetate extract. Their chemical structures were identified by MS and by UV and NMR spectroscopy.
Starting Page	173
Ending Page	178
Page Count	6
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	68
Issue Number	3-4
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	2008-07-04
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	High speed counter current chromatography 1,1-Diphenyl-2-picrylhydrazyl radical Selaginella moellendorffii Selaginellaceae Antioxidant Biflavone Measurement Science, Instrumentation Plant Sciences Biochemistry Pharmacy Organic Chemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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