NDLI: Automated microbore LC with peak compression for the assay of GR138950 and its active metabolite in human serum

Content Provider	Springer Nature Link
Author	Mills, M. J. Maltas, J. Lough, W. J.
Copyright Year	1997
Abstract	Microbore LC with peak compression sample injection can facilitate improved methods in LC. The advantages of this approach were clearly illustrated in the automated, microbore LC-peak compression assay of GR138950, a novel non-peptide angiotensin II antagonist selective for the AT1 receptor and its active metabolite in human serum. An existing analytical method had insufficient sensitivity to fully monitor the pharmacokinetic profile of GR138950 and its active metabolite (GR205838) when low doses were administrated. Consequently a robust assay with a lower limit of quantification was required. This was achieved by developing an alternative and fully automated solid phase extraction procedure with on-line peak compression sample injection onto a microbore (2.1 mm i.d.) column by means of a Zymate robot. This method produced cleaner extracts, improved precision, and lower limit of quantitation (1 ng mL−1) than had been achieved with the previous method which had used conventional LC.
Starting Page	275
Ending Page	283
Page Count	9
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	45
Issue Number	1
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	1997-01-01
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Column liquid chromatography Solid phase extraction Peak compression On-column focusing Microbore LC Analytical Chemistry Organic Chemistry Pharmacy Biochemistry Plant Sciences Measurement Science, Instrumentation
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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