NDLI: Comparison of HPLC Versus HPTLC-Densitometry for the Quantification of Chromone Glucosides in Saposhnikoviae divaricatae Radix

Content Provider	Springer Nature Link
Author	Scherübl, Rosmarie Manns, Detlef Heilmann, Jörg Franz, Gerhard
Copyright Year	2013
Abstract	A HPLC and a HPTLC-densitometric method were developed for the quantification of prim-O-glucosylcimifugin and 4′-O-β-d-glucosyl-5-O-methylvisamminol the major chromone glucosides in the roots of Saposhnikovia divaricata. The validation of both methods resulted in comparable parameters regarding stability, specificity, linearity, robustness, precision and recovery, whereas complementary advantages were obtained concerning LOD and LOQ. The HPTLC-based densitometry revealed a lower LOD (1.11 versus 4.37 μg mL−1 in HPLC) and LOQ (3.36 versus 13.24 μg mL−1 in HPLC) for prim-O-glucosylcimifugin, whereas the HPLC resulted in a lower LOD (1.00 versus 4.10 μg mL−1 in HPTLC-densitometry) and LOQ (3.04 versus 12.46 μg mL−1 in HPTLC-densitometry) for 4′-O-β-d-glucosyl-5-O-methylvisamminol. Both methods revealed nearly matching contents of the chromones after analysis of different commercially available batches of Saposhnikoviae divaricatae radix with a total content for both chromone glycosides in the range from 0.31 ± 0.011 to 0.56 ± 0.021 % determined by HPLC and between 0.34 ± 0.011 and 0.61 ± 0.009 % determined by HPTLC. The plant material cultivated in Germany showed a very similar content and ratio of both chromone glucosides in comparison to the standard batches originating from China.
Starting Page	1537
Ending Page	1543
Page Count	7
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	76
Issue Number	21-22
e-ISSN	16121112
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-07-26
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	HPTLC-densitometry HPLC Saposhnikovia divaricata Traditional Chinese medicine Chromone glucosides Quality control Chromatography Proteomics Pharmacy Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A Validated Quantification of Benzocaine in Lozenges Using TLC and a Flatbed Scanner

HPTLC measured values of primula saponins in extracts ofPrimulae radix. A comparison after derivatization between handspraying, dipping and autospraying

Comparative analysis of the constituents in Saposhnikoviae Radix and Glehniae Radix cum Rhizoma by monitoring inhibitory activity of nitric oxide production

UPLC versus HPLC on Drug Analysis: Advantageous, Applications and Their Validation Parameters

HPLC-DAD and TLC-Densitometry for quantification of hypericin inHypericum perforatum L. Extracts

Erratum to: UPLC versus HPLC on Drug Analysis: Advantageous, Applications and Their Validation Parameters

HPLC for Separation and Quantification of Deoxyribonucleic Acid Fragments and Measurement of Deoxyribonucleic Acid Degradation

Comparison of HPLC and MEEKC for Miconazole Nitrate Determination in Pharmaceutical Formulation

Development and Validation of a Method for Quantifying HER1 Extracellular Domain in Culture Supernatant by RP-HPLC

Comparison of HPLC Versus HPTLC-Densitometry for the Quantification of Chromone Glucosides in Saposhnikoviae divaricatae Radix

Similar Documents

A Validated Quantification of Benzocaine in Lozenges Using TLC and a Flatbed Scanner

HPTLC measured values of primula saponins in extracts ofPrimulae radix. A comparison after derivatization between handspraying, dipping and autospraying

Comparative analysis of the constituents in Saposhnikoviae Radix and Glehniae Radix cum Rhizoma by monitoring inhibitory activity of nitric oxide production

UPLC versus HPLC on Drug Analysis: Advantageous, Applications and Their Validation Parameters

HPLC-DAD and TLC-Densitometry for quantification of hypericin inHypericum perforatum L. Extracts

Erratum to: UPLC versus HPLC on Drug Analysis: Advantageous, Applications and Their Validation Parameters

HPLC for Separation and Quantification of Deoxyribonucleic Acid Fragments and Measurement of Deoxyribonucleic Acid Degradation

Comparison of HPLC and MEEKC for Miconazole Nitrate Determination in Pharmaceutical Formulation

Development and Validation of a Method for Quantifying HER1 Extracellular Domain in Culture Supernatant by RP-HPLC

Comparison of HPLC Versus HPTLC-Densitometry for the Quantification of Chromone Glucosides in Saposhnikoviae divaricatae Radix