NDLI: Derivatization, stability and chromatographic behavior ofo-phthaldialdehyde amino acid and amine derivatives:o-Phthaldialdehyde/ 2-mercaptoethanol reagent

Content Provider	Springer Nature Link
Author	Hanczkó, R. Molnár Perl, I.
Copyright Year	2003
Abstract	A literature overview is given of HPLC methods currently in use to determine amino acids (AAs) and amines (As) as theiro-phthaldialdyde (OPA)/2-mercaptoethanol (MCE) derivatives. This is followed by an exhaustive derivatization study performed using the classical proteinogen AAs, C1–C4 aliphatic As, several polyamines (PAs) and the OPA/MCE reagent. Model studies have been carried out as a function of reaction time and reagent composition. The primary importance of reagent composition (mole ratios of OPA to SH-group additive) is repeatedly proven. Stabilities of the OPA/MCE derivatives have been compared with corresponding OPA/3-mercaptopropionic acid (MPA) and/or OPA/N-acetyl-L-cysteine (NAC) compounds, obtained under the same conditions.Crucial factors proven to influence the stability of OPA derivatives of AAs and As are discussed in detail: (i) preparation and storage conditions of the OPA reagents, (ii) special behavior of the currently believed to be particularly unstable, AAs and As associated with their original molecular structure, e.g. glycine, β-alanine, γ-amino butyric acid (GABA), histidine, ornithine and lysine, several As, as well as (iii) the mole ratios of the OPA/SH-additive/AA and/or A.
Starting Page	S103
Ending Page	S113
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	57
Issue Number	1
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	2003-01-01
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Column liquid chromatography o-Phthaldialdehyde-amino acid and amine derivatives Stability studies and comparisons Analytical Chemistry Organic Chemistry Pharmacy Biochemistry Plant Sciences Measurement Science, Instrumentation
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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