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| Content Provider | Springer Nature Link |
|---|---|
| Author | Ning, Shujing You, Jinmao Sun, Zhiwei Zhang, Shijuan Ji, Zhongyin |
| Copyright Year | 2012 |
| Abstract | A simple and sensitive method for the determination of free aliphatic amines using 10-phenyl-acridone-2-sulfonyl chloride (PASC) as a labeling reagent by high-performance liquid chromatography with fluorescence detection and online mass spectrometry identification (HPLC-FLD-MS) has been developed. Derivatization conditions including reagent concentration, buffer pH, reaction time and temperature were optimized. PASC reacted with aliphatic amines at 50 °C for 4 min in aqueous acetonitrile (ACN) in the presence of sodiumtetraborate–NaOH buffer (0.10 mol L−1, pH 9.0) to give high yields of PASC-amine derivatives. Derivatives exhibited intense fluorescence with an excitation maximum at λex 265 nm and an emission maximum at λem 418 nm. The separation of derivatives was performed by a reversed-phase Hypersil BDS C8 column in combination with a gradient elution. The identification of derivatives was carried out by online post-column mass spectrometry with atmospheric pressure chemical ionization (APCI) source in positive-ion detection mode. Excellent linear responses were observed with the correlation coefficients of larger than 0.9997, and detection limits (at a signal-to-noise of 3:1) were from 3.0 to 24.3 fmol. Comparing with 10-ethyl-acridine-2-sulfonyl chloride (EASC), PASC exhibited more intense fluorescence and ultraviolet absorbance. The proposed method is sensitive and reproducible for the determination of aliphatic amines from water and soil samples. |
| Starting Page | 1107 |
| Ending Page | 1116 |
| Page Count | 10 |
| File Format | |
| ISSN | 00095893 |
| Journal | Chromatographia |
| Volume Number | 75 |
| Issue Number | 19-20 |
| e-ISSN | 16121112 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-08-15 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | HPLC/MS Aliphatic amines 10-Phenyl-acridone-2-sulfonyl chloride Pre-column derivatization Pharmacy Proteomics Laboratory Medicine Chromatography |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry |
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